钙调神经磷酸酶信号通路参与钙激动剂介导的心肌细胞肥大  

CALCINEURIN SIGNALING PATHWAY IS INVOLVED IN CARDIOMYOCYTE HYPERTROPHY MEDIATED BY CALCIUM ACTIVATORS

作　　者：杨永健[1] 张鑫[1] 周兴文[1] 朱俊[1] 赵龙生[1] 

机构地区：[1]成都军区成都总医院心血管内科,四川成都610083

出　　处：《中国应用生理学杂志》2001年第3期275-277,共3页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助课题 (3 9970 3 0 4)

摘　　要：目的 :在于探讨不同来源的细胞内游离钙 ([Ca2 + ]i)对钙调神经磷酸酶 (calcineurin ,CaN)依赖信号通路和心肌细胞肥大的影响。方法 :以原代培养的乳鼠心肌细胞为模型 ,血管紧张素Ⅱ(AngⅡ)、雷尼丁 (RY)和三磷酸肌醇 (IP3 ) (浓度均为 10 - 7mol/L)激活心肌细胞 [Ca2 + ]i,应用钙荧光指剂Fura 2 /AM动态观测心肌细胞 [Ca2 + ]i浓度 ,同时检测心肌细胞CaN活性及蛋白表达。用氚 亮氨酸 (3 H Leu)掺入量测定心肌细胞蛋白质合成速率。结果 :发现AngⅡ、RY和IP3明显增加心肌细胞 [Ca2 + ]i浓度、CaN活性及蛋白表达并提高3 H Leu的掺入量 ,与对照组心肌细胞相比差异显著 (P <0 .0 1)。结论 :激活心肌细胞 [Ca2 + ]i可明显提高心肌细胞蛋白合成速率 ,心肌细胞CaN活性及蛋白表达似与细胞[Ca2 + ]i变化有明显关系而与其来源无关 。Aim: To investigate the effects of intracellular free calcium([Ca 2+ ]i) from different resources on calcineurin(CaN) dependent signaling pathway and cardiomyocyte hypertrophy. Methods:Angiotensin Ⅱ(AngⅡ,10 -7 mol/L), ryanodine (RY, 10 -7 mol/L),inositol (1,4,5) trisphosphate (IP 3, 10 -7 mol/L) were applied to stimulate the [Ca 2+ ]i,CaN activity and protein expression were measured in primary cultured cardiomyocytes from wistar rat. [Ca 2+ ]i was measured using Fura 2/AM fluorescent technique. The incorporation of 3 H Leucine ( 3H Leu) determined the synthesis rates of protein.Results: AngⅡ, RY and IP 3 increased [Ca 2+ ]i,promoted CaN activity and protein expression, and 3H Leu incorporation in cultured cardiomyocytes compared with control ( P <0.05 or P <0.01). Conclsion:Cardiomyocyte hypertrophy is associated with the changes of cellular [Ca 2+ ]i, but not with its resources. Indicating that CaN signaling pathway play an important role in the hypertrophy of cardimyocytes.

关 键 词：钙调神经磷酸酶 心肌细胞肥大 信号转导 信号通路 钙激动剂 

分 类 号：R542.2[医药卫生—心血管疾病]