小毛莨内酯对结核病人颗粒裂解肽基因表达的作用  

Effects of Ternatolide on expression granulysin mRNAin the peripheral blood lymphocytes patients with mycobacterium tuberculosis

作　　者：詹莉[1] 戴华成 杨治平[1,3] 易著文[1] 舒平[1,4] 陈世明 

机构地区：[1]中南大学湘雅二医院儿科学教研室,长沙410011 [2]美国新泽西医学,牙学医学院药物分子生物学研究所 [3]湖南农业大学植物科学研究院,长沙410128 [4]华中科技大学同济医学院药学院,武汉430030 [5]湖南省结核病防治所,长沙410006

出　　处：《中国药理学通报》2001年第4期405-408,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题 No3930 0 139;No 396 70 334;No39970 32 6

摘　　要：目的　研究小毛莨内酯 (Ternatolide ,Tern)对健康成人 ,初诊未治、耐药肺结核病人及儿童结核患儿周围血淋巴细胞 (PBL)体外活化后颗粒裂解肽 (granulysin ,GLS)mRNA表达的作用 ,探索其抗结核菌的分子免疫学机理。方法　采用反转录聚合酶链反应半定量 (QC RT PCR)方法 ,测定不同剂量的Tern对以上 4组人群PBL颗粒裂解肽mRNA表达水平的影响。结果　当Tern在 10 0mg·L-1,2 0 0mg·L-1浓度时 ,均能诱导以上 4组人群体外活化的PBLGLSmRNA的表达 ,且各组诱导表达水平无显著差异。结论　Tern可能是通过提高CTL的效应分子GLSmRNA表达的水平 ,杀灭胞内致病菌MTB ,其诱导作用呈现剂量依赖性。AIM To study the effects of ternatolide(tern) on expression of granulysin mRNA in the activated peripheral blood lymphocytes(PBLS) of the adults and children with pulmonary Mycobacterium tuberculosis(PMTB). METHODS Using competitive quantitative reverse transcription polymerase chain reaction(QC TR PCR)analyze granulysin gene expression in the PBLs. RESULTS The dosage of 100 mg·L -1 , 200mg·L -1 of ternatolide significantly enhanced the granulysin mRNA experession in PBLs in vitro stimulated phytohemagglutinin (PHA) from adults and children with PMTB (P<0 001). The increase of granulysin mRNA expressing level in drug resistance MTB group no difference compared with no drug resistance group by stimulated tern (P>0 05). CONCLUSION The molecular mechanism of tern. against intracellular pathogens might be associated with the inducting highly level on the expression of granulysin mRNA, a anti bacterial peptide in activated human cytotoxic lymphocytes.

关 键 词：小毛莨内酯 猫爪草 颗粒裂解肽 QC-RT-PCR 结构 休眠菌 基因表达 

分 类 号：R978.3[医药卫生—药品] R52[医药卫生—药学]