PCR检定eNOS基因VNTR多态性  

Detection of the VNTR polymorphisms in eNOS gene by PCR method

作　　者：路萍[1] 吕星[2] 邢瑞云[2] 孙琪云[3] 钱瑞华[3] 邱泽武[3] 张少华[3] 洪权 郑晓飞[2] 

机构地区：[1]北京军医学院生物化学教研室,北京100071 [2]军事医学科学院放射医学研究所,北京100850 [3]军事医学科学院附属医院,北京100039

出　　处：《军事医学科学院院刊》2001年第3期171-173,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：国家"973"重点基础研究发展规划项目(G2 0 0 0 0 5 70 0 1)

摘　　要：目的 :建立eNOS基因VNTR多态性的PCR分析方法。方法 :按eNOS基因VNTR位点侧翼序列设计引物 ,自健康献血者基因组DNA扩增VNTR片段 ,琼脂糖凝胶电泳分析扩增片段的长度和数目 ,鉴定VNTR基因型。结果与结论 :在 2 0 8名健康献血者中鉴定出 6/ 5杂合、5 / 5纯合、5 / 4杂合和 4 / 4纯合 4种VNTR基因型 ;等位基因的分布频率与日本人相似 。ObjectiveTo establish the PCR method for analy si s and detection of the variable number of tandem repeats(VNTR)polymorphism in e~{!<~}WT~{!=~}NOS gene. MethodsWith the use of primers desig ned according to the flanking sequences of VNTR locus, VNTR fragments were amplified fro m genomic DNA of healthy subjects. For VNTR genotyping, the length and number of repeats were analyzed through agarose gel electrophoresis. Results and conclusionsFour genot ypes of VNTR, namely 6/5repeats heterozygous, 5/5repeats homozygous, 5/4repeats heterozygous and 4/4 repea ts homozygous, were identified from a total of 208 healthy subjects. The overall distribution of all ele frequencies was similar to that of Japanese, while it differed significantly from that of African Americans.

关 键 词：一氧化氮合酶 基因多态性 数目可变串联重复序列 聚合酶链反应 ENOS基因 

分 类 号：R346[医药卫生—基础医学]