肝脂消方对CCl_4诱导大鼠肝脂肪变性时SDHase、ATPase的影响  

Changes of SDHase and ATPase in Experimental Model Rats with Fatty Liver and Their Being Interfered by GanZhiXiao Formula(GZHXF)

作　　者：何东仪[1] 胡义扬[1] 刘平[1] 刘成[1] 薛惠明[1] 熊卫国[1] 李风华[1] 

机构地区：[1]上海中医药大学肝病研究所,上海200032

出　　处：《中国中西医结合消化杂志》2001年第3期133-135,138,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：上海市高等学校科学技术发展基金资助项目 (No.99C17)

摘　　要：目的 :观察肝脂肪变性时琥珀酸脱氢酶 (SDHase)、三磷酸腺苷酶 (ATPase)的变化 ,探讨肝脂消方抗肝脂肪变性的部分作用机制。方法 :CCl4高脂低蛋白饮食诱导大鼠肝脂肪变性 ,观察肝组织病理变化 ,检测甘油三酯、胆固醇的含量 ,并与正常组、东宝肝泰组对照。结果 :肝脂消方能提高模型组中降低的肝 SDHase和 ATPase的活性 ,显著抑制模型大鼠肝脂肪变性和肝内甘油三酯的沉积。结论 :调节三羧酸循环 ,促进脂肪酸的 β氧化和肝内甘油三酯的转运是肝脂消方抗肝脂肪变性的重要作用机制。Objective:To observe the changes of succinate dehydrogenase(SDHase)and adenosine triphosphatase(ATPase) in hepatic steatosis and to investigate some machanism of GZHXF resisting hepatic steatosis.Methods:Fatty liver model was induced by tetrachloride carbon(CCl 4) with high fat and low protein diet in rats.In the meantime Dongbao Gantai(DBGT) was used as control.After treatment,the various stained indexes such as liver hematoxylin and eosin(HE) staining,SDHase and ATPase histochemic staining,and the contents of triglyceride(TG) and total cholesterol(TC) were examined.Results:Hepatic steatosis was evident in model group,TG contents in liver increased,SDHase in liver decreased remarkably as well as ATPase.There was a antagonist action to the changes mentioned above in GZHXF.Conclusion:Regulation of tricarboxylic acid cycle,enhancement of fatty acid β oxidation and transportation of TG in liver could be important machanism of GZHXF to antagonize hepatic steatosis.

关 键 词：肝脂消方 脂肪肝 琥珀酸脱氢酶 三磷酸腺苷酶 中药 药理 

分 类 号：R285.5[医药卫生—中药学]