清肠栓对溃疡性结肠炎大鼠一氧化氮和一氧化氮合酶活性的影响  

Influence of Varied Doses Qingchangshuan on Activities of Nitric Oxide and Nitric Oxide Synthase in Rat with Ulcerative Colitis

作　　者：郑红斌[1] 胡鸿毅[1] 马贵同[1] 陈同钧[1] 

机构地区：[1]上海中医药大学附属龙华医院胃肠病研究室,,上海200032

出　　处：《中国中西医结合消化杂志》2001年第3期136-138,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

摘　　要：目的 :观察不同剂量清肠栓治疗溃疡性结肠 (U C)的作用及其对一氧化氮 (NO)和一氧化氮合酶 (NOS)活性的影响。方法 :将动物随机分成高、中、低剂量清肠栓组、SASP组、模型组、空白组 ,除空白组外其余 5组动物分别用三硝基苯磺酸 (TNBS)建立大鼠溃疡性结肠炎模型 ,连续用药 2周后取结肠组织 ,采用改良的 G法、分光光度法分别测定其 NO含量和 NOS活性。结果 :模型组大鼠 NO、NOS含量较空白组明显降低 ,其他各组均有不同程度的增高 ,尤其以清肠栓高剂量组的增高为明显。结论 :TNBS急性损伤使结肠组织的 NO、NOS活性发生改变 ,可能是 U C发生的重要机制。中药复方清肠栓具有调控 NO及 NOS活性的作用 ,是有效治疗 UC的可能机制之一。Objective:To investigate the influence of varied doses of Qingchangshuan(QCS) on activities of nitric oxide (NO) and nitric oxide synthase (NOS) in rat with ulcerative colitis (UC).Methods:Inflammatory model was induced in Wistar rats by trinitro benzene sulfonic acid (TNBS) injected into anus,and then treated with high dose,middle dose,low dose Qingchangshuan and salicylazosulfa pyridine (SASP) before the isolation of colon,respectively.Activity of NO and NOS were measured by means of spectrophotometry.Results:Activities of NO and NOS were significantly lower in model group than in normal,and increased when varied doses Qingchangshuan and SASP were used,especially in high dose group(P< 0.01 ).Conclusion:It suggests that the changes of activities of NO and NOS induced by TNBS may be a important mechanism of inflaming the colon in UC rat.The QCS has the function in adjusting to the levels of NO and NOS,therefore it can effectively treat the UC rat.

关 键 词：溃疡性结肠炎 一氧化氮 一氧化氮合酶 清肠栓 中医药疗法 

分 类 号：R259.746.2[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]