抗稻白叶枯病体细胞突变体筛选操作技术体系及其利用  

A Technical System for Screening Rice Somatic Cell Mutant Resistant to Xanthomonas oryzae

作　　者：孙立华[1] 朱作为[1] 汤邦根 王月芳[1] 吕学锋 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所 [2]湖北省长阳计委

出　　处：《江苏农业学报》1991年第1期32-37,共6页Jiangsu Journal of Agricultural Sciences

摘　　要：用对白叶枯病菌敏感的水稻品种的体细胞胚性细胞无性系,经继代培养,作为选择材料;以对数生长阶段的白叶枯病活体病原细菌为选择剂。已获得抗病突变体,其再生植株后代经人工接菌鉴定,具有稳定遗传抗病性。并且,该筛选技术体系具有重演性。从而建立了抗水稻白叶枯病的体细胞离体筛选体系。Rice somatic cell mutants resistant to Xanthomonas oryzae were screened in tissue culture and a technical system was set up. The embryogenic somatic cell clones were developed through subculture from 6 susceptible varieties-Nangeng 34, 729, 02428, Zhong-guo 91, and Nongken 57. The calli resistant to Xanthomonas oryzae were screened by culturing the pathogenic bacterium of logarithmic growth stage together with the embryogenic calli as a screening pressure. The progeny resistance of the regenerated plants was identified by inoculation. Among the 6 varieties 5 have obtained variants resistant to Xanthomona oryzae ( Table 1 ) , which indicates that the result can be repeated. In the resistant plant lines, some can resist different strains ( Table 2 ) , showing that a qualitative change has happened in disease resistance. The technical system is described in Fig. 1.

关 键 词：水稻 育种 白叶枯病菌 抗病突变体 

分 类 号：S511.103.4[农业科学—作物学]