盐逆境中无花果叶片蛋白质合成与脱落酸及脯氨酸积累的关系  

The Possible Relation between Protein Synthesis and Accumulation of Abscisic Acid and Free Proline in Fig Leaves under Salt Stress

作　　者：汪良驹[1] 王业遴[1] 刘友良 

机构地区：[1]南京农业大学园艺系

出　　处：《江苏农业学报》1991年第1期38-44,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金资助项目

摘　　要：盆栽无花果经150和300mM NaCl处理后,叶片组织内源脱落酸(ABA),脯氨酸和可溶性蛋白质含量均明显增加。经300mM NaCl处理1天后,可溶性蛋白质含量增加58.75％,2天后ABA增加到2.85倍,3天后游离脯氨酸增加13.06倍。在盐处理前,用转录抑制剂放线菌素D(CHD)或蛋白质合成抑制剂环已亚胺(CHI)处理无花果叶片,能明显抑制盐诱蛋白质,脱落酸和脯氨酸的积累,说明三种物质的积累依赖于基因活化和蛋白质合成。适当浓度的外源ABA可以促进叶片脯氨酸积累,而CHI或蛹虫草菌素均消除这种促进效应,说明ABA可促进无花果叶片中与脯氨酸合成有关的基因活化和蛋白质合成。上述结果说明,盐胁迫下,由于DNA的转录,引起脱落酸的合成,积累的脱落酸反过来促进基因活化和脯氨酸的积累。因而,蛋白质的合成是逆境激素及渗透调节的基础。The levels of soluble proteins, endogenous ABA and proline significantly increased in fig ( Ficus carica var. brunswick ) leaves treated with 150 and 300 mM NaCl solutions. When the fig leaves were immersed in solutions of actinomycin ( a DNA transcription inhibitor ) or cycloheximide ( a protein synthesis inhibitor in cytoplasmic ribosomes ) for 5 minutes, the accumulation of salt-induced soluble proteins,ABA and free proline disappeared, showing that the accumulation might depend upon gene activation and protein synthesis. Exogenous ABA of certain concentrations promoted leaf proline accumulation under or without salt stress, but this effect might be eliminated by cycloheximide or cordycepin ( an inhibitor of mRNA chain prolongation ) , showing that ABA might promote gene activation and protein synthesis responsible for proline synthesis in fig leaves. The results suggested that salt stress stimulated DNA activation. Due to DNA transcription, ABA was biosynthesized, and the increased ABA promoted gene activation and proline accumulation. Thus salt-induced proteins might be the basis of plant hormone regulation and osmore-gulation under salt stress.

关 键 词：盐胁迫 无花果 叶片 蛋白 脱落酸 

分 类 号：S663.301[农业科学—果树学]