中药促进大鼠脊髓修复再生的超微结构研究  

ULTRASTRUCTURE STUDY OF SPINAL CORD RESTORATION BY CHINESE HERB (SCI) IN RATS

作　　者：马育平[1] 张平[2] 苏静[2] 高秀来[1] 常超英[2] 陆涛[1] 韩风岳[2] 

机构地区：[1]首都医科大学人体解剖学教研室,北京100054 [2]中国中医研究院骨科研究所

出　　处：《解剖学杂志》2001年第4期364-368,共5页Chinese Journal of Anatomy

基　　金：北京市教委科研基金资助课题 (NO 97KJ)

摘　　要：目的 :观察脊髓损伤后 ,脊髓神经毡等超微结构的变化和中药脊髓Ⅰ号对大鼠损伤脊髓的保护作用和促修复再生作用。方法 :制备下胸髓右侧半横断损伤的Wistar大鼠模型 ,用透射电镜观察和分析 ,脊髓Ⅰ号对脊髓损伤区神经组织超微结构的影响 ,探讨其与受损神经组织修复再生的关系。结果 :(1 )在空白对照组大鼠 ,脊髓损伤区神经元胞体、轴突和树突野的超微结构发生明显的变性。服用脊髓Ⅰ号可以保护脊髓损伤区的神经元 ,并且促进受损神经元胞体、神经纤维和树突野的修复再生 ;(2 )脊髓Ⅰ号组大鼠 ,脊髓星形胶质细胞和毛细血管周细胞的反应比较轻微。结论 :脊髓损伤后及时服用脊髓Ⅰ号 ,可以有效地保护脊髓损伤区的神经组织 ,抑制神经胶质和周细胞的增生反应 ,创造有利于神经元再生的环境。Ultrastructure alterations of spinal neuropile were observed after spinal cord injury,and the effect of the spinal cord granule I (SCI,a granule of Chinese Herbs) on the alterations were also observed.Methods:Forty-five Wistar rats were divided into 4 groups:1 SCIG:After the spinal hemisection,12 rats were administrated with SCI from one day to a month;2 BYTG:12 rats were administrated with Buyanghuanwutang (as control Chinese Herb) from one day to a month;3 MPSG:9 rats were treated with methylprednisolone from one day to 15 days;4.BCG:12 rats were administrated with NS from one day to a month;in addition,there was NCG:3 normal controls with uninjured the spinal cord.For groups 1-4,12 rats were subdivided into 4 subgroups:the rats were treated for 1,3,15,and 30 days respectively,there was no 30 day-subgroup in the MPSG.The injured areas were observed with TEM.These electromicrophotographs were analyzed with Leica Q550CW image analysis system to delineate the relation between effect of SCI and repair-regeneration of spinal neurons.Results:1.There was obvious ultrastructure degeneration of spinal neuron,axon and dendrite profile in BCG.It has been observed that the protection for spinal neurons and regeneration of the perikaryon,axon and dendrite profile was distinct in rat treated with SCI.2.Degree of reaction of the glia and pericytes to spinal injury was slight in rats-SCIG.Conclusion:After spinal cord injury and administration with SCI,the spinal tissue can distinctly avoid secondary damage,and reactive proliferation of glia and pericytes is inhibited,these are microenvironment adapted for regeneration of spinal neurons.

关 键 词：脊髓 脊髓损伤 再生 中药脊髓Ⅰ号 脊髓神经毡 作用机理 

分 类 号：R285.5[医药卫生—中药学] R269.512[医药卫生—中医学]