大鼠肺巨噬细胞iNOS电镜细胞化学定位显示法  

The demonstration of NOS in rat alveolar macrophage with electron microscopic NADPH-d cytochemistry

作　　者：张宇新[1] 卢鹤翔[1] 高俊玲[1] 孙树勋[1] 

机构地区：[1]华北煤炭医学院基础医学部,唐山063000

出　　处：《电子显微学报》2001年第3期248-250,共3页Journal of Chinese Electron Microscopy Society

摘　　要：目的　对大鼠肺泡巨噬细胞NOS进行细胞内定位显示。方法　由大鼠支气管肺泡灌洗液中获取肺泡巨噬细胞 ,富集制备成单层细胞爬片。在 37℃ ,5%CO2 的二氧化碳培养箱中 ,用LPS ( 1g L)刺激 2 4h以诱导肺泡巨噬细胞NOS表达。采用NADPH d电镜细胞化学术对肺泡巨噬细胞NOS进行定位显示。结果　活化的AM胞浆内可见大量颗粒状电子密度沉淀。结论　NADPHObjectives:This study was designed to localize the nitric oxide synthase (NOS) in rat alveolar macrophages (AM) in vitro. Methods:Special techniques of bronchoalveolar lavage were used to harvest wistar rat AM. After cells enrichment, AM on glass cover slips were cultured 24h with LPS (1g/L) or saline (control group) at 37℃ in 5% CO 2 incubator. The AM monolayers were then stained with electron microscopic NADPH diaphorase (NADPH d) cytochemistry. Results:The activated AM demonstrated that electron dense grains were throughout cytoplasmic area. Conclusions:With NADPH d cytochemistry, the AM inducible NOS is a kind of cytoplasmic enzyme.

关 键 词：肺泡巨噬细胞 NOS NADPH-d电镜细胞化学 大鼠 定位显示 AM胞浆 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]