细胞核凋亡过程中核基质的变化  

The change of nuclear matrix lamina in apoptosis of nucleus

作　　者：赵允[1] 沈延[1] 吴旻[1] 翟中和[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《电子显微学报》2001年第2期87-92,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目!(3980 0 0 75;19890 380 );国家重大基础研究资助项目!(G19990 5 390 5 )

摘　　要：在非细胞体系中以细胞色素C作为诱导剂 ,诱导小鼠肝细胞核发生凋亡。在透射电子显微镜及扫描电子显微镜下观察到凋亡的细胞核呈现典型的凋亡形态学特征。整装电镜的结果显示 ,在凋亡过程中核基质结构被破坏 ,但核基质结构仍然存在 。We induced apoptosis of mouse nuclei in cell free system using cytochrome C. The apoptotic nucleus displayed the typical apoptotic character when it was observed under transmission electron microscope and scanning electron microscope. The results of whole mount electron microscopy indicated that nuclear matrix still existed during the course of apoptosis, whereas lamina system was destroyed. It was nuclear matrix that connected the apoptotic bodies and apoptotic nucleus, which laid the structural foundation of apoptotic bodies.

关 键 词：细胞色素C 小鼠肝细胞核 凋亡 核基质 细胞凋亡 透射电镜 扫描电镜 

分 类 号：Q255[生物学—细胞生物学] Q－336