离子束注入胸腺嘧啶的紫外、红外效应研究  

An Ultraviolet and Infrared Spectroscopic Study of Ion-Implanted Thymine

作　　者：谷运红[1] 秦广雍[1] 霍裕平[1] 卞坡[1] 王卫东[1] 苏明杰[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,郑州450052

出　　处：《光散射学报》2001年第1期39-44,共6页The Journal of Light Scattering

摘　　要：本文中我们利用紫外、红外光谱等分析手段获取低能离子束生物诱变的信息。发现用30 ke V低能氮离子和氩离子在 0 - 2× 1 0 16 ions/cm2注入剂量范围内注入胸腺嘧啶 ,其残存率随剂量的变化趋势均出现了不同于γ射线等辐照时的线性或肩型曲线 ,而呈现一种新的变化趋势——“马鞍型”曲线 ,表明参与结构的质量沉积不足以解释“马鞍型”曲线。红外光谱的分析结果表明在离子束注入过程中胸腺嘧啶分子确实存在结构上的修复。研究结果表明紫外、红外光谱可以给出离子束注入前后生物分子结构突变或重组、分子结构突变与注入剂量的关系以及分子的修复等信息。Ultraviolet spectroscopy and infrared spectroscopy wereused to study the biological changes of molecules induced by low energy ion beam implantation. The effect of different doses of 30KeV low energy N + and Ar + ions implanted on thymine was studied. The dose dependence of thymine by both N + ions and Ar + ions implantation is not usual but shows a peculiar saddle shape. This special dose dependence cannot be fully explained by the amount mass precipitant. Infrared spectroscopic result indicates the presence of annealing of thymine during ion beam implantation. The current study shows that ultraviolet and infrared spectroscopy are good methods to obtain information about structural changes, annealing and the relation between structural changes and dose of the ion implantation in biological systems.

关 键 词：低能离子束注入 胸腺嘧啶 “马鞍型”曲线 紫外光谱 红外光谱 生物诱变 注入剂量 分子修复 分子结构 

分 类 号：Q617[生物学—生物物理学] O434[机械工程—光学工程]