应用单细胞微凝胶电泳技术检测成纤维细胞的放射敏感性  

USE OF SINGLE CELL MICROGEL ELECTROPHORESIS TO DETECT THE CELLUAR RADIOSENSITIVITY OF TWO FIBROBLAST STRAINS

作　　者：徐永忠[1] 郑斯英[1] 朱巍[1] 赵经涌[1] 曹建平[1] 

机构地区：[1]苏州大学核医学院放射医学系

出　　处：《辐射研究与辐射工艺学报》2001年第3期186-190,共5页Journal of Radiation Research and Radiation Processing

基　　金：国防科工委基金 (Y5 5 732 6 2 )

摘　　要：应用单细胞微凝胶电泳 (SCGE)技术以及噻唑蓝 (MTT)比色法检测辐射诱发的成纤维细胞系AT5BIVA和GM6 37的初始DNA双链断裂数及断裂后的修复与细胞放射敏感性之间的关系。结果表明 ,AT5BIVA细胞系的放射敏感性显著高于GM 6 37。两种细胞系的初始DNA双链断裂数均随剂量的增加而增加 ,呈显著的剂量效应关系 ,在给定的剂量点 ,辐射诱发的AT5BIVA细胞系的初始DNA双链断裂数显著高于GM 6 37。AT5BIVA细胞系对辐射诱发的DNA双链断裂的修复能力小于GM 6 37。结果提示 ,辐射诱发的初始DNA双链断裂数及断裂后的修复与细胞的放射敏感性均有一定的相关性 。Single cell microgel electrophoresis(SCGE) and MTT assay were used to determine the relationships between radiation-induced initial DNA double-strand breaks(DSBs), the ability of cells to repair DSBs and cellular radiosensitivity in both AT5BIVA and GM637 cell lines. The results demonstrated that AT5BIVA cell line was significantly more radiosensitive than GM637 . The initial DSBs in both AT5BIVA and GM637 lines increased with the rise of irradiation dose, and showed significant dependence on dose. At a given dose of 20Gy, radiation-induced initial DSBs in AT5BIVA cell line was signficantly higher than those in GM637. The ability of AT5BIVA cell line to repair DSBs was more powerful than that of GM637 . The results suggested that the radiation-induced initial DSBs in cells and the ability of cells to repair DSBs both correlated with cellular radiosensitivity to a certain extent, and could be used as potential predictors of intrinsic radiosensitivity of cells.

关 键 词：单细胞微凝胶电泳 DNA双链断裂 修复 放射敏感性 成纤维细胞 放射生物学 

分 类 号：Q691[生物学—生物物理学] R811[医药卫生—放射医学]