骨形成蛋白对小鼠造血型急性放射损伤治疗作用的研究  

EFFECT OF BMPs ON HEMATOPOIETIC INJURY OF ACUTE RADIATION SICKNESS IN MICE

作　　者：田琼[1] 张绍章[1] 蒲勤[2] 张发科[1] Hannah X.H. 

机构地区：[1]第四军医大学基础部教学实验中心,西安710032 [2]第四军医大学生化教研室,西安710032 [3]香港科技大学生物化学系

出　　处：《辐射研究与辐射工艺学报》2001年第3期199-204,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金 (39970 2 31)

摘　　要：小鼠经60 Coγ射线 6 .5 - 7.5Gy照射后 ,分别于肌袋内植入纯化牛BMP ,或腹腔内注入可溶性rhBMP - 2m ,或移植Pbk -hBMP - 2 /NIH3T3入腹腔内 ,观察动物的 30d活存情况 ,并检测几种造血参数。结果表明 ,pbBMP(纯化牛骨形成蛋白 )、hBMP - 2m (重组骨形成蛋白 - 2多肽 )Pbk -BMP - 2 /NIH3T3cellline(PBKhBMP - 2转基因细胞株 )对小鼠 6 .5、7.0、7.5Gyγ射线照射后急性放射造血损伤有治疗作用 :GM -CFU集落形成率、各项造血参数和 30d活存率均较对照组有显著性差异 (p <0 .0 1)。本工作结果提示 ,BMP对成年动物放射所致造血损伤具有治疗作用 ,其机理与促进已损伤的造血功能的修复作用有关。To investigate the effect of bone morphogenetic proteins (BMPs) on the acute hematopoietic sickness induced in mice. BMP, rhBMP-2m and PBK/ hBMP-2 -NIH3T3 cells were prepared as previously discribed . In this study, the effect of BMPs on hematopoiesis included some hematological parameters. The survival ratio on the 30th d post irradiation and formation of bone marrow CFU-GM colomies were detected. The experiments indicate that pbBMP (purified bovine bone morphogenetic protein) could increase the formation of bone marrow CFU-GM colomies ( p <0.05 ) at 10 th d after irradiation. rhBMP-2m increased the survival rate of mice irradiated by 7.5Gy. All mice in control group died in 30days, while 10%,15% and 35% mice survived when they were injected ip with 0.5mg, 1.0mg and 2.0mg of rhBMP-2m respectively. All hematological parameters of treated mice were significantly higher than those of control group ( p <0.01). PBK/ hBMP-2 -NIH3T3 cells were established and transplanted into mice irradiated by 7.0Gy γ ray by i.p. The survival r atio of treated mice higher than that of negative control group ( p <0.01), and all hematopoietic parameters were increased statistically significantly ( p <0.01). These data supported our hypothesis: BMPs can be used for the tratement of the acute radiation sickness. The results indicated that in adult mice , BMPs could recover the hematopoietic injury from acute radiation sickness.

关 键 词：BMP 放射损放 造血修复 骨形成蛋白 急性放射病 

分 类 号：R818.05[医药卫生—放射医学] R818.74[医药卫生—临床医学]