牛单个腔前卵泡切片制作效果观察  

Effect of Microsection Manufacture from Bovine Single Preantral Follicle

作　　者：高建明[1] 高立云[1] 赵耀[1] 吴学清[1] 

机构地区：[1]北京农学院动物科技系,北京102206

出　　处：《草食家畜》2001年第2期22-23,共2页Grass-Feeding Livestock

基　　金：北京市自然科学基金资助项目! (编号 :5972 0 0 5)

摘　　要：本实验以牛离体卵巢上获得的腔前卵泡为材料 ,对单个腔前卵泡切片制作的效果进行了观察。实验结果表明 ,将腔前卵泡包埋入琼脂中 ,并进行HE整染 ,可减少腔前卵泡的丢失 ,有利于比较清晰地观察其形态特征 ,其观察率为 90 .36% ,而对照组仅为 8.89%。另外 ,切片前染色处理时间对卵泡观察效果也有一定的影响 ,以 32min好于 52min和 2 7min。The bovine single preantral follicle of ovaries from slaughterhouse were disposed and wrapped up in agar gel.In control group,the follicles disappeared easily because of the same color between the follicles and agar gel.However,in experiment group,this problem was resolved thoroughly.The results showed that the observing rates of follicles were 90.36% in the experiment group,but the data were only 8.89% in the control group.Moreover,before secting,the dyeing time of 32 min was better than that of 52 min and 27 min.

关 键 词：牛 腔前卵泡 切片 制作 卵母细胞 质量评定 染色体处理时间 HE整染 

分 类 号：S852.162[农业科学—基础兽医学]