玉米细胞质线粒体DNA RFLP分类研究  

RFLP Classification of Cytoplasmic mtDNA in Maize

作　　者：李小琴[1] 万邦惠[2] 刘纪麟[1] 李建生[1] 郑用琏[1] 徐尚忠[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,湖北武汉430070 [2]华南农业大学农学系,广东广州510642

出　　处：《作物学报》2001年第4期476-481,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金重点资助项目;广东省自然科学基金资助

摘　　要：本实验用 4个酶、 4个探针组成 16个酶 /探针组合对玉米 N、T、C、S、WBMs、 80 1CMS等细胞质进行了线粒体 DNA( mt DNA) RFL P分析。一方面对玉米细胞质 mt DNA RFL P分类方法进行研究 ,证明只要酶 /探针技术体系合适 ,可以通过该方法对细胞质进行快速准确地分类 ;提出探针的选择是主要的 ,酶次之 ;认为 Pst /B30、H ind /p Bcm H3、Bam H /p HJ2 - 7- 1三个酶 /探针可以作为 mt DNARFLP分类技术体系。另一方面 ,用 mt DNA RFL P分类法把新发现的 WBMs不育胞质划分为 S组 ,80 1CMS划分为RFLP analysis of mtDNA was carried out with 16(4×4) combinations of enzyme/probs to study the N T, C, S cytoplasmics of maize, as well as the uncertain cytoplasmics of a male sterils lines, WBMs and 801 Ms. The results indicated: (1) RFLP analysis of mtDNA could classify the cytoplasm of maize quickly and correctly if appropriate enzyme/probe. Combination(s) was(were) used. (2) Option of probes was of primary importance with that of enzymes ranking the second . (3) Three enzyme/probe combinations, i. e., Pst Ⅰ/B30, Hin dⅢ/pBcmH 3 and Bam HⅠ/pHJ2 7 1, were suggested as better technical systems for the classification of the cytoplasmics of maize with RFLP mtDNA analysis. (4) The male Sterile Cytoplasmics of WBMs and 801 Ms were classified as S and C cytoplasms respectively according to results of RFLP mtDNA analysis.

关 键 词：RFLP 线粒体DNA 细胞质分类 雄性不育 玉米 T型小斑病 

分 类 号：Q942[生物学—植物学]