1株中国人HCV-2a型基因组部分cDNA序列的种系发生树分析  

Phylogenetic Analysis of Partial cDNA Sequence of Hepatitis C Virus Derived from a Patient with HCV Infectinn in China

作　　者：霍艳英[1] 徐德忠[1] 赵小宁[1] 李如琳[1] 杨海 杨国琦 

机构地区：[1]第四军医大学流行病学教研室,西安710032 [2]西安市卫生防疫站

出　　处：《中国公共卫生》2001年第9期769-771,共3页Chinese Journal of Public Health

摘　　要：用反转录套式PCR(RT -PCR)法从陕西地区 1例NANB型肝炎患者血清中扩增出部分E区的cDNA片段 ,克隆于PGEM -T载体中用全自动序列分析仪进行双向测序 ,测得的序列以及已发表的同一分离株 5′NRC、C区序列 ,分别同Genbank中注册的 3 6株已知基因型的HCV相应区段的序列比较 ,构建种系发生树。结果　 5′NCR区相应序列构建的种系发生树表明该分离株为 2型 ,而用C区和E区构建的种系发生树均表明该分离株为 2a型。提示该分离株为 2a型 ;对同一病原体来说 ,其基因区段的变异度不同 ,则种系发生树分析的结果不完全相同 ,变异度大的结果较准确。Objective To clone and sequence partial cDNA of Hepatitis C virus(HCV) genome of Shanxi China,constructed phylogenetic tree.Methods A 878bp fragment in envelope region was amplified by reverse transcription nested polymerize chain reaction (RT-PCR).The fragment was cloned into PGEM T-vector,then sequenced by auto-sequencing instrument.Phylogenetic tree were constructed from the 5′NCR sequene of 252bp,C sequence of 436bp and E sequence of 878bp by HCV-XA and other 36 strains registered in Genbank respectively.Results Phylogenetic tree constructed from the 5' NCR indicates HCV-XA belonged to type 2;from C and E indicate it belonged to subtype 2a.Conclusion The isolated strain belonged to type 2a.The variation of the subgenome affected the result of phylogenetic analyses for the same pathogen.

关 键 词：丙型肝炎病毒 病毒基因 种系发生树 CDNA序列 

分 类 号：R373.2[医药卫生—病原生物学]