BCL-2蛋白在燃煤型砷中毒患者皮肤中的表达分析  

Analysis on Experssion of BCL-2 Protein in Skin of Patient with Arseniasis by Coal-burning

作　　者：胡昌军[1] 张爱华[1] 黄晓欣[2] 任渝江[2] 

机构地区：[1]贵阳医学院预防医学系 [2]中国人民解放军第44医院

出　　处：《中国公共卫生》2001年第9期777-778,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助 (39660 0 70 )

摘　　要：目的　研究BCL - 2基因在燃煤型砷中毒致皮肤恶性病变过程中的作用。方法　将患者分为癌变组 (A组 ,18例 )、癌前组 (B组 ,11例 )和一般病变组 (C组 ,3 9例 ) ;BCL - 2蛋白的检测用免疫组化两步法。结果　A、B及C 3组的阳性细胞密度分别为 ( 4 5 97± 14 2 9)、( 3 3 2 0± 15 16)和 ( 2 2 74±15 3 8) ,3组阳性率分别为 94 4 4 %、90 91%和 5 8 97% ,二者3组间差异均有显著性 (A与C :P <0 0 1) ,B与C :P <0 0 5 ) ;3组阳性细胞分级构成差异亦有显著性 (P <0 0 1) ,A组Ⅲ级占 5 0 % ,而B、C两组仅分别为 9 0 9%和 2 5 6%。结论　在燃煤型砷中毒致皮肤癌变过程中 ,BCL - 2基因起积极作用 ,其检测对该病的良、恶性鉴别有实用价值。Objective To study the effect of BCL-2 gene on skin carcinogensis due to arseniasis caused by coal-burning.Methods Patients with arseniasis caused by coal-buring were divided into carcinoma group(A group,18 cases),pre-carcinoma group(B group,11 cases) and common pathological changes group(C group,39 cases);immu-histochemistry of two steps was used to determine BCL-2 protein.Results Among A?B and C group,the positive cell density were(45.97±14.29),(33.20±15.16) and (22.74±15.38);the positive percentages were 94.44%?90.91% and 58.97% respectively.Whether positive cell density or percentage,the differences had statistical significance between A and C groups(P<0.01) or B and C group(P<0.05).The difference of grade constitution also had statistical significance(P<0.01);grade Ⅲ made up 50% in A group,but 9.09% in B group and 2.56% in C group.Conclusion BCL-2 gene may take a positive effect on the development of the disease;its detection has pratical value for judging whether the focus of skin due to this disease is malignant or benign.

关 键 词：燃煤型砷中毒 病变皮肤 BCL-2蛋白 免疫组织化学 

分 类 号：R599.9[医药卫生—内科学] R135.1[医药卫生—临床医学]