用PCR扩增法检测耐甲氧西林葡萄球菌的mecA基因  

Detection of MecA Gene in Methicillin-resistant Staphylococcus

作　　者：侯晓娜[1] 丁雪松[1] 于笑难[1] 杨婧[1] 解立威[1] 任微[1] 

机构地区：[1]沈阳军区总医院,沈阳110016

出　　处：《中国公共卫生》2001年第9期847-848,共2页Chinese Journal of Public Health

摘　　要：目的　应用PCR扩增法检测耐甲氧西林葡萄球菌(MRS)的mecA基因。方法　临床分离的 161株葡萄球菌 ,应用PCR扩增法鉴定MRS的mecA基因 ,并与Oxacillin纸片扩散法进行比较。结果　 161株葡萄球菌用Oxacillin纸片扩散法和mecA基因法检测MRS有 4株菌有差异 ,mecA基因法鉴定阴性而Oxacillin纸片扩散法鉴定为耐药株 ,3株纸片扩散临界耐药 ,mecA基因法鉴定阳性 ,两种方法的符合率是96 89%。结论　mecA基因检测技术可以准确、快速判定MRS ,特别是在鉴定纸片扩散法临界耐药株具有优势。Objective To establish a method of polymerose chain reaction(PCR) for identification of clinical staphylococcal isolates.Methods A total of 161 clinical staphylococcal isolates were tested to identify mecA gene in Methicillin-Resistant Staphylococcus(MRS) by PCR and Oxiacillin disk diffusion method.Results Among the 161 staphylococcal isolates,there were four strains negative for mecA gene by PCR,while resistant by disk diffusion.Three of 161 MRS strains showed borderline resistance.All three strains were tested positive for mecA by PCR.The consistence of two methods was 96.89%.Conclusion The technology of PCR to rapid identification of MRS was superior to disk diffusion method especially for borderline-resistant strains.

关 键 词：MECA基因 耐甲氧西林葡萄球菌 纸片扩散 聚合酶链反应 

分 类 号：R113[医药卫生—公共卫生与预防医学] R378.11