体外转化生长因子-β_1与表皮生长因子对人前列腺上皮细胞增殖的作用调节  

The regulation effect of transforming growth factor-β_1, epithelial growth factor on the proliferation of human prostatic epithelial cell in vitro

作　　者：王建业[1] 左涛[1] 万奔[1] 黎健[2] 朱刚[1] 刘明[1] 张耀光[1] 

机构地区：[1]卫生部北京医院泌尿外科,100730 [2]北京老年医学研究所生化室

出　　处：《中华老年医学杂志》2001年第4期255-258,共4页Chinese Journal of Geriatrics

摘　　要：目的　体外观察转化生长因子 β1(TGF β1)、表皮生长因子 (EGF)对前列腺上皮细胞增殖的调节作用。　方法　胶原酶消化组织 ,建立原代前列腺上皮细胞系 ,细胞培养于无血清培养基中。前列腺特异抗原 (PSA)、Pan Keratin等免疫组织化学染色鉴定培养细胞。绘制细胞生长曲线和运用细胞增殖ELISA染色 (BrdU)方法观察传代后细胞的生长状况及不同剂量的生长因子TGF β1、EGF对细胞增殖的作用。　结果　培养细胞具有典型的上皮细胞形态特征 ,PSA、Pan Keratin染色阳性 ,细胞传代 5～ 7次后开始衰退。不同培养基条件可明显地影响细胞生长活性。EGF促进细胞增殖活性 ,TGF β1则抑制增殖 ,其作用与剂量成正比。此外 ,EGF与TGF β1相互减弱对方的作用。　结论　体外培养的人前列腺上皮细胞增殖受生长因子TGF β1和EGF调节 ,结果提示TGF β1、EGF可能在前列腺增生 (BPH)Objective To investigate the effect of TGF β 1 and EGF on the proliferation of cultured human prostatic epithelial cells. Methods The primary culture was prepared by collagenase digestion of minced prostatic tissue obtained from prostatectomies of 8 benign prostatic hyperplasia(BPH) patients (aged 53 to 70) and 5 organ donors (aged 23 to 36). Cells were cultured in serum free prostatic epithelial cell growth media and identified by immunocytochemical method. The cells were passaged and cultured in media without bovine pituitary extract (BPE) and then, EGF and TGF β 1 with different concentration were added into the culture either alone or in combination according to the experimental protocol. The cell proliferation was assessed by counting the cell number (BrdU) and ELISA. Results Cultured cells showed typical morphological features of epithelium and stained positive for PSA and Pan Keratin. After 5 7 passages cells went senescence. BPE and EGF enhanced whereas TGF β 1 inhibited the cell proliferation and the inhibition was reversed by increasing the concentrations of EGF. The effect of both TGF β 1 and EGF displayed concentration dependent manner. Conclusions Cell proliferation of the cultured human prostatic epithelial cells could be modulated by TGF β 1 and EGF. These results indicated that TGF β 1 and EGF, as the negative and positive growth factors might play an important role in the pathogenesis of BPH.

关 键 词：前列腺增生 生长因子 细胞培养 转化生长因子-Β1 表皮生长因子 细胞增殖 

分 类 号：R697.32[医药卫生—泌尿科学]