钙调蛋白及其基因表达与先天性心脏病右心室舒张性心力衰竭发病关系的研究  

Molecular Mechanisms Underlying Calcium Handling in Right Ventricular Diastolic Heart Failure

作　　者：钟明[1] 张运[2] 张薇[2] 卞继峰[3] 卜培莉[2] 赵静[2] 

机构地区：[1]山东医科大学附属医院干部保健科,山东省济南市250012 [2]山东医科大学附属医院心内科,山东省济南市250012 [3]山东医科大学分子生物学实验室,250012

出　　处：《中国循环杂志》2001年第4期252-254,共3页Chinese Circulation Journal

基　　金：国家自然科学基金资助课题(39970294)

摘　　要：目的：探讨先天性心脏病右心室舒张性心力衰竭（ＤＨＦ）患者心肌细胞内Ｃａ２＋超负荷、钙调蛋白及其基因表达的变化。　　　　方法：采用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）和Ｗｅｓｔｅｒｎｂｌｏｔ技术测定１０例先天性心脏病右心室ＤＨＦ患者（ＤＨＦ组）和６例正常对照（对照组）钙调蛋白及其基因的表达。　　　　结果：ＤＨＦ组患者心肌细胞内Ｃａ２＋含量较对照组高３倍以上，有非常显著性差异（Ｐ＜０．０１）；肌浆网钙－三磷酸腺苷酶（ＳＲ　Ｃａ２＋ＡＴＰａｓｅ）和细胞膜Ｌ型Ｃａ２＋通道的信使核糖核酸（ｍＲＮＡ）水平较对照组减低，有显著性差异（Ｐ均＜０．０５），而肌浆网磷酸受纳蛋白、兰尼碱受体和肌集钙蛋白的ｍＲＮＡ表达较对照组无显著性差异；ＳＲ　Ｃａ２＋ＡＴＰａｓｅ蛋白的相对含量较对照组减低，有显著性差异（Ｐ＜０．０５＝；磷酸受纳蛋白的相对含量无显著性差异。　　　　结论：细胞膜Ｌ型Ｃａ２＋通道和ＳＲ　Ｃａ２＋－ＡＴＰａｓｅ基因表达减低是导致先天性心脏病心肌细胞内Ｃａ２＋超负荷和右心室ＤＨＦ发生的主导因素。Objective:To elucidate molecular mechani sms underlying calcium handling in patients with right ventricular diastolic hea rt failure(RVDHF)from mRNA level and pro tein expression. Methods:Ten patients with RVDHF and 6 control subjects were enrolled in this s tudy. The mRNA amounts of these calcium handling genes were measured by reverse transcriptase polymerase chain reaction (RT-PCR)and normalized to the mRNA level s of actin.The protein levels of sarcopl asmic reticulum (SR)Ca2+- adenosine t riphosphatase(ATPase)and phospholamban w ere analyzed by western blot analysis. Results:The content of calcium was sig nificantly increased in patients with RV DHF(p<0.01=.RT-PCR analyses showed that the steady-state level of mRNA encoding the SR Ca2+-ATPase and cardiac L-type calcium channel were decreased significantly in patients with RVDHF(p<0.05).In contrast,no alterations of the mRNA levels for SR phospholamban,ryanodine receptor,calsequestrin were observed in these patients.Protein level of SR Ca2+-TPase was significantly reduced in patients with RVDHF,whereas protein level of phospholamban was unchanged. Conclusion:The expressions of L-type c alcium channel and SR Ca2+-ATPase wer e down regulated in patients with RVDHF. These changes may be a contributory fact or for RVDHF.

关 键 词：心力衰竭 右心室舒张性 钙调蛋白 先天性心脏病 基因表达 

分 类 号：R541.6[医药卫生—心血管疾病] R541.1[医药卫生—内科学]