缺氧缺血后海马组织c-Fos基因表达与细胞凋亡  

C-fos induction in hippocampus after neonatal hypoxia-ischemia

作　　者：丁艳洁[1] 汤云珍[1] 李海浪[1] 蒋犁[1] 

机构地区：[1]东南大学附属中大医院儿科,南京210009

出　　处：《江苏医药》2001年第8期581-582,共2页Jiangsu Medical Journal

摘　　要：目的　研究缺氧缺血后海马组织中c Fos基因的表达及效应。方法　利用新生儿缺氧缺血模型 ,采用RT PCR技术与DNA电泳方法 ,观察缺氧缺血后海马组织中即刻早基因c FosmRNA的表达 ,及后期的细胞凋亡。结果　对照组海马c Fos的mRNA低水平表达 ,亦未能检测到细胞凋亡 ;实验组缺氧缺血后 0小时 ,1小时可见c FosmRNA明显升高 (P <0 0 5 ) ,2小时后逐渐回至基础水平。于损伤 5天后DNA电泳出现Ladder ,提示大量细胞发生凋亡。结论　缺氧缺血导致海马细胞内c Fos一过性表达上调 ,可能是导致后期海马细胞凋亡的机制之一。Objective To explore the expression of c Fos gene and the effects of such expression in neonatal rats’hippocampus after hypoxia ischemia.Methods RT PCR and DNA electrophoresis were used to detect the expression of c Fos and the cell apoptosis followed in hippocampus of hypoxia ischemia neonatal rats.Results The expression of c Fos mRNA in hippocampus was lower in controls,and cell apoptosis couldn’t be detected in the later time;but c Fos mRNA remarkably increased at 0h,1h after hypoxia ( P <0 05),then returned to the basal level after 2h.The DNA ladder could be detected after 5d post hypoxia,showed that the cell apoptosis in the hippocampus increased distinctly.Conclusion Hypoxia ischemia can induce the transient expression increase of c Fos gene in the hippocampal cells,and such expression may be involved in the mechanisms of hippocapal cells apoptosis days later.

关 键 词：脑缺氧 脑缺血 新生儿 海马组织 C-FOS基因表达 实验研究 细胞凋亡 

分 类 号：R722.12[医药卫生—儿科]