核心天线CP43、CP47的荧光光谱特性  

FLUORESCENCE SPECTRAL PROPERTIES OF CORE ANTENNAS CP43 AND CP47

作　　者：贺俊芳[1,2] 王水才[1] 张舒[1,2] 贺锋涛[1,2] 任兆玉[1,2] 单际修[3] 李良壁[3] 匡廷云[3] 

机构地区：[1]中国科学院西安光学精密机械研究所瞬态室 [2]西北大学,西安710069 [3]中国科学院植物所

出　　处：《光子学报》2001年第8期933-937,共5页Acta Photonica Sinica

基　　金：国家重点基础研究专项基金 (G1 9980 1 0 1 0 0 );所长基金资助项目&&

摘　　要：采用快速扫描成象光谱技术对核心天线 CP43和 CP47的荧光光谱特性进行研究 ,获取了它们的积分荧光谱 ,通过积分荧光谱的组分光谱解析 ,并结合吸收光谱分析认为 CP43和CP47具有这样的 Chl a的光谱特性 ,CP43 :Chl a6 6 2 .4 3 6 6 0 、Chl a6 70 .2 3 6 6 9、Chl a6 84 .0 26 82 ,CP47:Chl a6 6 4.916 6 0 、Chl a6 71.716 6 9、Chl a6 81.3 56 80 、( Chl aea,a代表吸收峰 ;e代表发射峰 ) ;另外长波长组分 6 94.86 nm、70 2 .3 4 nm( CP43 )、6 96 .0 2 nm( CP47)可能是由吸收 >6 90 nm的 Chl a分子所产生 ;CP43与CP47相比还存在有 Chl a6 76 .3 26 75,但是还没有看到 CP43具有 6 75nm吸收谱带的报道 .对 CP43和 CP47的荧光光谱分析 ,认为 CP47中的 Chl a6 6 9nm分子团和 Chl a6 80 nm分子团间的能量传递比 CP43中 Chl a6 6 9nm和 Chl a6 82 nm分子团的能量传递更为有效 ;β-Car与 Chl a分子结合状态在 CP47中要比 CP43中紧密 .Rapid scanning imaging spectroscopy was used to focus on core antennas CP43 and CP47.Their integral fluorescence spectra were detected and fitted with multiple component spectra.On the basis of the fluorescence spectra,component spectrum resolution and absorption spectra,it was concluded that CP43 contained the spectra properties of Chl a:Chl a 662.43 660 ,Chl a 670.23 669 ,Chl a 684.02 682 and CP47 Chl a 664.91 660 ,Chl a 671.71 669 ,Chl a 681.35 680 ,(Chla e a,a is the peak of absorption,e is the peak of emission).In addition,the possible origins of long wavelength components 694.86nm(CP43),702.34nm(CP43),696.02nm(CP47) were Chl a molecules absorbing long wavelength (>690nm) light.Compared with CP47,CP43 also contained one component Chl a 676.32 675 .But the reports about the absorption peak at 675nm haven′t been found.The analysis of fluorescence spectra of CP43 and CP47 shown that the energy transfer between Chl a669nm molecule pool and Chl a 680nm molecule pool in CP47 was more efficient than between Chl a 669nm and Chl a 682nm in CP43,and the conjunct state of β-Car and Chl a molecules in CP47 is closer than in CP43.

关 键 词：核心天线 CP43 CP47 荧光光谱 快速扫描成象光谱技术 积分荧光谱 光合作用光系统 

分 类 号：Q945.11[生物学—植物学]