黑质损毁的大鼠双侧尾壳核多巴胺及其代谢产物的高效液相色谱-电化学测定  

HPLC-EC DETERMINATION ON THE CONTENTS OF DOPAMINE AND ITS METABOLITES IN BILATERAL STRIATA OF SUBSTANTIA NIGRA LESIONED RATS

作　　者：熊国祥 吴晋宝[1] 周斯舜 秦月琴[1] 

机构地区：[1]上海第二医科大学解剖学教研室,上海200025

出　　处：《解剖学报》1991年第4期395-398,共4页Acta Anatomica Sinica

摘　　要：正常及用6-羟基多巴胺(6-OHDA)损毁黑质的28只大白鼠分成3组,用高效液相色谱-电化学检测器(high performance liquid chromatography-electrochemical detection,HPLC-EC)分别测定其两侧尾壳核中多巴胺(DA)及其代谢产物高香草酸(HVA)、3,4-二羟基苯乙酸(DOPAC)的含量。单侧损毁组损毁侧尾壳核中DA、HVA/DA、DOPAC/DA 3项指标,分别相当于健侧的22.27％、420.00％、199.75％,显示损毁侧纹状体内幸存的DA神经末梢有代偿性的递质释放增强效应。单侧损毁组损毁侧与双侧损毁组两侧尾壳核间、单侧损毁组健侧与正常对照组两侧尾壳核间,上述各指标差异无显著性,提示损毁侧尾壳核内代偿性的多巴胺释放增强效应,很可能是同侧黑质-纹状体系统的机能代偿所致。The contents of dopamine (DA) and its metabolites, homovanillic acid (HVA) and 3,4-dihydroxyphenyl acetic acid(DOPAC), in both striata were determined with HPLC-EC technique in each of 28 albino rats, which were divided into 3 groups (bilateral lesioned, unilateral lesioned and healthy control groups). Among the unilateral lesioned rats, three indices such as DA, HVA/DA and DOPAC/DA in the lesioned striata were 22.27％, 420.00％ and 199.75％ of those of the intact striata, respectively. This suggests that there may be an enhanced compensatory DA release in the striatum ipsilateral to the lesion of the rat. Having compared the lesioned striata of unilateral lesioned rats with those of bilateral lesioned animals, and the intact striata of unilateral lesioned rats with those of the control ones, we did not find any significant differences in DA, HVA/DA or DOPAC/DA. The result implies that the compensatory DA release in the lesioned striatum probably accounts for the mechanism of the ipsilateral nigrostriatal system, not for the crossed nigrostriatal fibers.

关 键 词：黑质 纹状体 多巴胺 高香草酸 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]