β-葡萄糖醛酸苷酶与母乳性黄疸关系的观察  

Correlation between beta glucuronidase and breast milk jaundice

作　　者：朴梅花[1] 李在玲[1] 李莉[1] 罗凤珍[1] 

机构地区：[1]北京大学第三医院儿科,100083

出　　处：《中华儿科杂志》2001年第6期327-329,共3页Chinese Journal of Pediatrics

摘　　要：目的　动态观察母乳、新生儿粪便中 β 葡萄糖醛酸苷酶 (β glucuronidase ,β GD)活性变化的规律及其与血清胆红素浓度的关系。方法　以纯母乳喂养的 18例母乳性黄疸患儿和 12例生理性黄疸儿为观察对象。于生后第 1、2、3和 4周测定母乳及新生儿粪便中 β GD活性和血清胆红素浓度。结果　母乳中 β GD活性在母乳性黄疸组第 1、2、3、4周 (中位数 ,M)分别为 178、10 2、134和 91kU/L ,生理性黄疸组分别为 32 0、12 8、182、16 4kU/L ,两组各期差异无显著性 (P >0 0 5 ) ;新生儿粪便中 β GD活性在母乳性黄疸组分别为 (5 12± 2 91)、(6 6 0± 15 3)、(6 2 9± 2 82 )和 (6 39± 2 37)U/g ,生理性黄疸组分别为 (6 72± 118)、(5 82± 2 70 )、(5 73± 2 11)和 (714± 15 3)U/g ,两组差异亦无显著性 (P >0 0 5 )。母乳性黄疸组血清胆红素浓度与母乳及粪便中酶活性的相关系数分别为 0 2 2 4和 0 0 2 7(P >0 0 5 ) ;生理性黄疸组分别为 0 10 9及 0 140 (P >0 0 5 ) ,两组均无相关性。结论　βObjective To observe dynamical changes of beta glucuronidase activity in breast milk and infant feces, and to explore the correlation with the breast milk jaundice. Methods The study was conducted on 30 breastfed healthy, full term newborns. Babies with hyperbilirubinemia not associated with breast milk jaundice or physiologic jaundice were excluded. The breast milk jaundice group (study group) consisted of 18 babies. The physiologic jaundice group (control group) consisted of 12 babies. The beta glucuronidase activity was estimated in the breast milk and infant feces. Samples were collected at the 1st, 2nd, 3rd and 4th postnatal weeks. The beta glucuronidase assay was performed by using phenolphthalein mono beta glucuronic acid substrate (Sigma Kit No:325) and results were expressed as modified Sigma units/ml (U/ml) and modified Sigma units/g(U/g). The serum total bilirubin concentration was determined at the same time points.Results (1) Serum bilirubin concentrations at the 1st, 2nd, 3rd and 4th postnatal weeks were (207±46), (166±44),(167±68) and (136±55)μmol/L, respectively in the study group, and were (148±38), (107±47), (68±27) and (43±19)μmol/L, respectively in the control group ( t =3.37, 3.20, 4.76 and 5.54, respectively, P <0.01). (2) Breast milk beta glucuronidase activities at each time points were 178, 102, 134 and 91 kU/L, respectively in the study group, and were 320, 128, 182 and 164 kU/L, respectively in the control group ( t =1.53, 1.35,1.29 and 1 34, respectively, P >0.05). (3) Infant fecal beta glucuronidase activities at each time points were (512±291), (660±153), (629±282) and (639±237) U/g, respectively in the study group, and were (672±118), (582±270), (573±211) and (714±153) U/g, respectively in the control group ( t =1.69, 0.87, 0.56 and 0.89, respectively, P >0.05). (4) Serum total bilirubin levels did not show any correlation to the beta glucuronidase activities both in breast milk and infant feces in the study group (correlation coefficients

关 键 词：黄疸 新生儿 葡萄醛酸酶 β-GD 母乳性黄疸 

分 类 号：R722.17[医药卫生—儿科]