小型猪冠状动脉球囊扩张术后MAPKs活性及c-fos mRNA增加  

MAPKs activity and c-fos mRNA in vascular tissues increased after coronary artery balloon injury in swine

作　　者：何昆仑[1] 盖鲁粤[1] 黄大显[1] 刘乃奎[2] 唐朝枢[2] 

机构地区：[1]中国人民解放军总医院心内科,北京100853 [2]北京医科大学心血管病基础研究所,北京100034

出　　处：《中国病理生理杂志》2001年第6期546-549,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :观察小型猪冠状动脉球囊扩张术后丝裂素活化蛋白激酶 (MAPKs)活性及c -fosmRNA的变化。方法 :小型猪 17头 ,6头作为正常对照 ,其余小型猪左冠状动脉前降支和回旋支行球囊过度扩张术。静脉麻醉处死动物 ,通过病理染色切片分析目标血管组织的形态学变化 ;采用反转录 -聚合酶链反应 (RT -PCR)方法 ,定量检测目标血管组织c-fosmRNA的表达 ;并测定血管组织MAPKs活性。结果 :球囊扩张术后 3天血管组织MAPKs活性及c -fosmRNA的表达均明显增加 (5 1 5 % ,P <0 0 1;318 7% ,P <0 0 1) ,术后 30d出现新生内膜的增厚和管腔狭窄。结论 :早期MAPKs活性升高和cAIM: To observe the changes in mitogen-activated protein kinases (MAPKs) activity and gene expression of c-fos after coronary artery balloon injury in swine. METHODS: Six of the seventeen Chinese swine were as control group, and the others underwent coronary angioplasty to LAD or CLx. The animals were sacrificed at three and thirty days following the procedure. The cross-sections were stained hematoxylin-eosin, strichrome, and Verhoef-van Giesen after the target segments were dissected free from the hearts, and the morphologic characteristics were investigated by computer-assistant analysis system. The target segments were also processed to examine the gene expression of c-fos by reverse transcription-polymerase chain reaction (RT-PCR) and to measure the activity of MAPKs by biochemistry. RESULTS: MAPKs activity and gene expression of c-fos in the dilated segments were significantly higher than that of normal segments three days after coronary balloon injury (51.5%, P< 0.01;318.7%, P< 0.01). Maximal neointima thickness is seriously increased and the lumen perimeter is deeply restricted after the initial procedure. CONCLUSION: The early increases in MAPKs activity and c-fos mRNA may be involved in the restenosis process after coronary balloon injury.

关 键 词：血管成形术 蛋白激酶 癌基因 冠状动脉 MAPKS C-FOS MRNA基因 

分 类 号：R543.3[医药卫生—心血管疾病] R732.21[医药卫生—内科学]