海水浸泡对火器伤兔肢体骨骼肌组织脂质过氧化反应影响的研究  

The influence of seawater on lipid peroxidation of the muscle tissue wounded by firearm in rabbit limbs

作　　者：陈蕾[1] 赖西南 陈志强[1] 张良潮[1] 

机构地区：[1]重庆第三军医大学大坪医院野战外科研究所,重庆400042

出　　处：《中国病理生理杂志》2001年第6期556-558,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :观察海水浸泡对火器伤伤口组织脂质过氧化反应的影响。方法 :以滑膛枪发射质量 2 5 0mg钢珠 ,致伤兔后肢 ,伤后将致伤兔随机分为两组 :一组为海水浸泡组 (SIG) ,将致伤兔浸泡于粗制海盐配制的人造海水中 30min ;另一组为单纯致伤组 (SWG) ,伤后不浸泡海水。伤后 3、6、12、2 4h手术取距伤道边缘 0 5cm(A区 )、1 5cm(B区 )和 2 5cm(C区 )处肌组织 ,测定ATP、丙二醛 (MDA)含量和超氧化物歧化酶 (SOD)活力。取伤前骨骼肌组织作为对照。结果 :伤后SIG兔伤肢骨骼肌MDA含量明显高于对照 ,伤后 3、6h升高 ,12h略降 ,2 4h有再次升高的趋势。SWG的MDA含量变化与SIG相似 ,但升幅低于SIG ;2组SOD活力和ATP含量的改变与MDA含量变化相似。结论 :海水浸泡的火器伤伤口脂质过氧化反应增强 。AIM: The influence of the seawater on the lipid peroxidation of the muscle tissue wounded by firearm was studied. METHODS: Both two limbs of rabbits were wounded by the steel ball of 250 mg. The wounded rabbits were divided into two group: One is seawater immersed group (SIG), which was dipped in artificial seawater for 30 min. Another is the single wounded group (SWG), which wasn't dipped in seawater. Then, muscle tissues 0.5 cm (A area), 1.5 cm (B area) and 2.5 cm (C area) from wound edge were cut for biochemical analysis at 3, 6, 12, 24 h of post-wound, respectively. The contents of ATP, MDA and SOD activity of all these tissues were detected, pre-wounded muscle tissues were used as control group. RESULTS: The content of MDA in SIG increased obviously at 3, 6 h, respectively, but it decreased slightly at 12 h and increased again at 24 h. The change in MDA in SWG is similar to that in SIG but more slightly. The changes in both the activity of SOD and the content of ATP in two groups is similar to that of MDA. CONCLUSION: Seawater induced more serious lipid peroxidation in the muscle tissue wounded by firearm. [

关 键 词：创伤 枪 海水 过氧化脂质类 火器伤 骨骼肌组织 

分 类 号：R826.5[医药卫生—临床医学]