孕妇血清、乳汁及新生儿脐血中输血传播病毒感染的研究  

Study on infection of transfusion transmitted virus in serum, breast milk of pregnant women and umbilical venous blood from their newborns

作　　者：方芳[1] 仲伟国[2] 王华梁[1] 王爱华[1] 赵琳[1] 古航[2] 

机构地区：[1]第二军医大学附属东方肝胆外科医院实验诊断科,上海200438 [2]第二军医大学附属长海医院妇产科,上海200438

出　　处：《中华妇产科杂志》2001年第6期330-332,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　了解输血传播病毒 (TTV)在孕妇血清、乳汁及新生儿脐血中的感染状况 ,探讨TTV的传播途径。方法　用半巢式聚合酶链反应方法对 15 0例孕妇血清、乳汁及新生儿脐血标本 ,进行TTVDNA扩增 ,对 8例PCR阳性扩增产物再进行直接测序。结果　 (1) 15 0例孕妇血清TTVDNA的阳性率为 11 3% (17/ 15 0 ) ,17例孕妇血清TTVDNA阳性者中 ,乳汁阳性 11例 ,其新生儿脐血均为阴性。 (2 )检测 3例孕妇血清TTVDNA序列及乳汁TTVDNA序列 ,其核苷酸同源性为 97 6 %～ 99 2 % ;5例孕妇血清TTVDNA序列之间同源性为 71 5 %～ 92 8% ,与日本原型株N2 2的核苷酸及氨基酸序列同源性为 71 5 %～ 92 8%。结论　孕妇血清及乳汁中存在TTV感染 ,经乳汁传播可能是该病毒感染的主要方式之一。Objective To study transfusion transmitted virus (TTV) infection in pregnant women and their newborns, and the route of transmission. Methods Serum, breast milk from 150 pregnant women and umbilical venous blood from their newborns were collected. TTV DNA was amplified by semi nested polymerase chain reaction, and the positive products were sequenced directly. Results The positive rate of TTV DNA in pregnancy serum was 11 3% (17/150). Among the 17 serum positive cases, TTV DNA was detected in 11 breast milk samples, and none in all the umbilical blood. The homologous of partial nucleotide sequence of TTV DNA between the 3 serum samples and their breast milk were 97 6%～99 2%. Compared the 5 serum positive samples (A1～A5) with N22 segments of Japanese original isolate, the homologous of partial nucleotide and amino acid sequence were 71 5%～92 8%, 71 5%～92 8%, respectively. Conclusion There exists TTV infection in pregnancy serum and breast milk. Transmission by breast feeding may be one of the main pathway causing TTV infection.

关 键 词：DNA病毒感染 乳汁 疾病传播 胎血 新生儿 TTV 

分 类 号：R446.5[医药卫生—诊断学]