ELISA测定血清可溶性HLA-I类抗原  

Quantitation of serum soluble HLA class I antigens by enzyme-linked immunosorbent assay

作　　者：裴斌 姚芳娟[2] 杨珏琴[2] 许玲娣[2] 葛瑜[2] 范丽安[2] 

机构地区：[1]厦门市中心血站,厦门361004 [2]上海第二医科大学上海市免疫学研究所

出　　处：《临床检验杂志》2001年第4期213-215,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的　建立定量测定可溶性HLA I类抗原的ELISA方法。方法　测定上海地区汉族正常人群血清可溶性HLA I类抗原的含量 ,并进一步研究可能影响这一含量的因素。结果　测得 116例上海地区汉族正常个体血清可溶性HLA I类抗原的含量为 5 2 9 3 2±2 82 88ng/ml。结论　性别和常见HLA A位点抗原型别对血清可溶性HLA I类抗原的含量未造成显著影响。Objective\ To establish a method to quantitative soluble HLA class I antigens and to detect the concentration of serum soluble HLA class I antigens in Shanghai Chinese population of Han nationality Methods \ The method to quantitative soluble HLA class I antigens is sensitive,specific and reliable Results\ The concentration of serum soluble HLA class I antigens in 116 Shanghai Han nationality Chinese individuals is 529 32±282 88 ng/ml Conclusions\ Sex and HLA A antigens of individuality did not affect the concentration of serum soluble HLA class I antigens significantly

关 键 词：HLA-Ⅰ类抗原 可溶性抗原 酶联免疫吸附测定 定量测定 血清诊断 

分 类 号：R446.11[医药卫生—诊断学] R446.62[医药卫生—临床医学]