联合应用生长激素和谷氨酰胺对短肠大鼠小肠粘膜吸收功能的影响  

EFFECT OF COMBINATION TREATMENT WITH GROWTH HORMONE AND GLUTAMINE ON INTESTINAL MUCOSAL ABSORPTIVE FUNCTION IN RATS WITH SMALL BOWEL SYNDROME

作　　者：谢建新[1] 顾岩[2] 刘银坤 赵淑民[1] 罗宝国[1] 陈丽琏[1] 吴肇汉[2] 左焕琛[1] 

机构地区：[1]复旦大学医学院人体解剖学教研室 [2]上海中山医院普外科,上海200032 [3]上海中山医院肝癌研究所,上海200032

出　　处：《解剖学杂志》2001年第3期231-234,共4页Chinese Journal of Anatomy

基　　金：国家教委博士点基金 (No.973 6);上海市卫生局科技发展基金 (No.974 2 9)资助

摘　　要：目的 :探讨联合应用生长激素和谷氨酰胺对短肠大鼠小肠粘膜吸收功能的影响。材料和方法 :研究选用 40只手术成功的SD短肠大鼠 ,按 2× 2析因实验设计随机分为四组 ,分别给予常规全肠外营养、附加谷氨酰胺、附加生长激素及附加谷氨酰胺和生长激素全肠外营养 ,持续 6天。应用生化酶学的方法测定各组小肠粘膜上皮Na+ ,K+ ATPase的活性。结果 :谷氨酰胺组小肠粘膜Na+ ,K+ ATPase的活性较常规组明显增高 ;生长激素组Na+ ,K+ ATPase的活性无明显增高 ;而联合应用谷氨酰胺和生长激素组小肠粘膜上皮Na+ ,K+ ATPase的活性较常规组亦有显著性增高。结论 :研究表明联合应用谷氨酰胺和生长激素能够有效的增加小肠粘膜上皮Na+ ,K+ AT Pase的活性 ,从而提高其吸收功能。To explore the effect of combination treatment with growth hormone and glutamine on intestinal mucosal absorptive function. Methods: Forty Sprague-Dawley rats with small bowel syndrome were randomly divided into 4 groups according to 2×2 factorial experiment and were treated with standard PN(STD group, n=10), PN+Gln(Gln group, n=10), PN+GH(GH group, n=10) and PN+Gln+GH(GG group, n=10) respectively for 6 days. Contrasting studies on Na +, K +-ATPase activity of mucosal epithelia of residual small bowel among these groups was done by biochemical enzymology method. Results: Na +, K +-ATPase activity of intestinal mucosal epithelia in Gln group was significantly increased than that in the STD group(P<0.01), Na +, K +-ATPase activity in GH group was not significantly increased as compared with that in STD group, but Na +, K +-ATPase activity of intestinal mucosal epithelia in the GG group was significantly increased as compared with that in STD group(P<0.01). Conclusion: Combined GH and Gln enriched parenteral nutrition can efficiently increase Na +, K +-ATPase activity of intestinal mucosal epithelia and enhance its absorptive function.

关 键 词：小肠 肠外营养 谷氨酰胺 生长激素 小肠粘膜吸收功能 

分 类 号：R459.3[医药卫生—治疗学]