哮喘豚鼠模型支气管平滑肌钙通道特性的变化  

Changes of Ca^(2+) channel behaviors in bronchial smooth muscle cells of asthmas guinea-pig model

作　　者：王忠慧[1] 马忠森[1] 周及红[2] 李昌辉[1] 张文杰[3] 陈冬梅[4] 赫国志[1] 

机构地区：[1]白求恩医科大学第二临床学院呼吸科,长春130041 [2]白求恩医科大学第二临床学院放射科,长春130041 [3]白求恩医科大学第二临床学院生理教研室,长春130041 [4]白求恩医科大学第一临床学院心血管科

出　　处：《中华结核和呼吸杂志》2001年第9期539-541,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：吉林省科委基金资助项目 ( 970 5 73)

摘　　要：目的　探讨钙离子通道变化与支气管哮喘豚鼠模型气道高反应性的关系。方法　利用膜片钳技术细胞贴附式方法测定哮喘A组 (4只 ,2 2个细胞 )和正常对照A组 (5只 ,2 5个细胞 )支气管平滑肌细胞 (BSMCS)电压依赖性钙离子通道动力学变化 ,全细胞式测定哮喘B组 (5只 ,2 5个细胞 )和正常对照B组 (6只 ,2 4个细胞 )BSMCS 内向峰值钙电流。结果　 (1)BSMCS 钙通道开放时间哮喘A组为 (1 70± 0 40 )ms,正常对照A组为 (0 70± 0 10 )ms,两组开放时间比较差异有显著性 (P <0 0 1) ;哮喘A组BSMCS 钙通道关闭时间为 (5 7± 0 6 )ms ,正常对照A组为 (7 9± 0 4)ms,两组比较差异有显著性 (P <0 0 1) ;开放概率哮喘A组为 (0 40± 0 10 ) % ,正常对照A组为 (0 2 0± 0 2 0 ) % ,哮喘A组较正常对照A组增加了 46 % ,两者比较差异有显著性 (P <0 0 1) ;(2 )哮喘B组BSMCS 平均内向峰值钙电流为 (- 5 4± 2 3)PA(微微安培 ) ,正常对照B组为 (- 2 5± 8)PA ,哮喘B组较正常对照B组增加了 1 17倍 ,两组比较差异有显著性 (P <0 0 1)。结论　哮喘豚鼠BSMCS 钙离子通道活性增加 。Objective To investigate the relationship between the change of calcium channel behavior in bronchial smooth muscle cells (BSMC S) and the airway hyperresponsiveness in guinea pigs with asthma Methods The changes of calcium channel dynamics in BSMC S of 4 asthma guinea pig models (22 cells) and 5 normal guinea pigs (25 cells),and the inner peak calcium current in BSMC S of 5 asthma guinea pig models (25 cells) and 6 normal guinea pigs (24 cells) were deter mined by patch clamp techniques Results (1)The open time of calcium channel in BSMC S of asthma group was (1 70±0 40) ms, 2 32 times as much as that of control group (0 70±0 10) ms; the close time of calcium channel in BSMC S of asthma group (5 7±0 6) ms, was significantly shorter that that of control group (7 9±0 4) ms; The open probability in asthma group (0 40±0 10)% was significautly increased than that of control group (0 20±0 20)%, ( P <0 01); (2)The inner peak calcium current in BSMC S of asthma group was (-54±23)PA, 1 17 times as muth as that of control group (-25±8) PA, ( P <0 01) Conclusion The enhanced activity of calcium channel an BSMC S of asthma guinea pig mudels may be one of the important factors responsible for the airway hyperresponsiveness

关 键 词：哮喘 支气管平滑肌细胞 钙通道 气道高反应性 

分 类 号：R562.25[医药卫生—呼吸系统]