红霉素、磷霉素对铜绿假单胞菌生物被膜体外作用的研究  

Effects of erythromycin and fosfomycin on Pseudomonas aeruginosa biofilm in vitro

作　　者：徐志豪[1] 刘富光[1] 王选锭[1] 原双晋[1] 李丽红[1] 

机构地区：[1]浙江大学医学院附属第二医院呼吸科,杭州310009

出　　处：《中华结核和呼吸杂志》2001年第6期342-344,I001,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　通过体外铜绿假单胞菌 (PA)生物被膜 (BF)的培养、诱导和观察 ,研究非抗假单胞菌药物红霉素 (EM)和磷霉素 (FOS)对BF的作用。方法　选取临床分离呼吸道PA和质控菌株ATCC2 785 3,采用胰酶大豆肉汤 (TSB teflon)系统和生理盐水 (NS teflon)系统进行BF的培养和诱导 ,扫描电子显微镜观察BF ,微量稀释法测定药物的最低抑菌浓度 (MIC)。结果　经过 1、3、7d的孵育 ,临床分离菌的BF在TSB teflon系统中 ,质控菌株在NS teflon系统中均随着时间延长而增多 ,而且 1mg/L红霉素和 2mg/L磷霉素可以在孵育过程中抑制BF的形成 ,MIC测定提示 1mg/L红霉素和 2mg/L磷霉素与头孢他啶或左氧氟沙星联合对BF型铜绿假单胞菌有协同抗菌活性。Objective To observe the effects on Pseudomonas aeruginosa biofilm of non antipseudomonal drugs such as erythromycin and fosfomycin Methods Clinical isolates of Pseudomonas aeruginosa in respiratory infection and ATCC27853 were cultivated in TSB Teflon and NS Teflon system respectively for biolfilm formation Biofilm was observed under scanning electronic microscope (SEM) MICs were determined by broth micro dilution Results SEM showed that biofilm of clinical isolate in TSB Teflon system and ATCC27853 in NS Teflon system increased as being cultivated for longer in 1, 3 and 7 days And biofilm formation in the process of cultivating was inhibited by SubMIC of erythromycin and fosfomycin MICs evaluation suggested that there were synergetic effects against biofilm Pseudomonas aeruginosa when SubMIC of erythromycin and fosfomycin were combined with ceftazidime or levofloxacin in vitro Conclusion SubMIC of erythromycin and fosfomycin could inhibit Pseudomonas aeruginosa biofilm in vitro

关 键 词：铜绿假单胞菌 生物被膜 红霉素 磷霉素 

分 类 号：R56[医药卫生—呼吸系统]