豌豆根瘤菌共生基因在S.fredii中的表达及共生质粒间的相互作用  

The Expression of Symbiotic Genes of Rhizobium Leguminosarum bv.Viciae 1229 in Sinorhizobium Fredii and Interaction Between two Symbiotic Plasmids

作　　者：缪礼鸿[1] 周俊初[1] 

机构地区：[1]华中农业大学农业部农业微生物重点实验室,湖北武汉430070

出　　处：《武汉大学学报（理学版）》2001年第4期498-502,共5页Journal of Wuhan University:Natural Science Edition

基　　金：欧盟 INCO-DC合作研究计划 ERBIC18CT970 191课题经费资助

摘　　要：通过两亲本接合转移 ,将豌豆根瘤菌 ( Rhizobium leguminosarum bv. viciae) 12 2 9的约 2 5 5 0 kb的共生质粒 p Sym12 2 9分别转入费氏中华根瘤菌 ( Sinorhizobium fredii) HN0 1SR及其消除了共生质粒的菌株 HND10 0中 .在人工培养条件下 ,用 1 4C标记的薄层层析 ( TL C)技术检测转移接合子的结瘤因子 ( L COs)的结果表明 ,转移接合子 HND10 0 ( p Sym12 2 9)产生的 L COs与豌豆根瘤菌 12 2 9相同 .植物盆栽结瘤试验结果表明 ,HND10 0( p Sym 12 2 9)能够在豌豆品种 Nigra上形成白色球状的无效根瘤 ,而豌豆根瘤菌 12 2 9则形成正常的长圆柱状的有效根瘤 .将 p Sym 12 2 9转入 HN 0 1SR后 ,部分接合子表现出与 HND10 0 ( p Sym 12 2 9)相同的结瘤性状 ,而另一部分接合子的结瘤性状与 HN0 1SR相同 ,显示了 HN0 1SR与 12 2 9两菌株异源共生质粒之间结瘤功能的抑制关系 .The symbiotic plasmid of Rhizobium leguminosarum bv.viciae 1229 was transferred into Sinorhizobium fredii HN01SR and its pSym cured derivatives HND100. The transconjugant HND100(pSym1229) expressed the same LCOs as that of R. leguminosarum 1229 in free-living condition detected by TLC plate. The results of pot plant tests indicated that HND100(pSym1229) could only form many small ineffective nodules on Vicia sativa cultivar Nigra on which R. leguminosarum 1229 formed long cylindroid nitrogen-fixing nodules. The transconjugant HN01SR(pSym1229) which contained whole pSym1229 also formed ineffective nodules on Nigra, but fail to form nodules on soybean. The results suggest the repression of nodulation function between symbiotic plasmids of R. leguminosarum 1229 and S. fredii HN01SR.

关 键 词：共生质粒 费氏中华根瘤菌 结瘤因子 豌豆根瘤菌 基因表达 接合子 

分 类 号：Q786[生物学—分子生物学] Q939.114