灰树花子实体多糖和菌丝体多糖的比较分析  

Analysis of Polysaccharide Isolated from the Fruit Bodies and Mycelia of Grifola frondosa

作　　者：冯慧琴[1] 杨庆尧[1] 杨晓彤[1] 糜可[1] 祝凌云[1] 

机构地区：[1]上海师范大学微生物与免疫学研究所,上海200062

出　　处：《华东师范大学学报（自然科学版）》2001年第3期91-96,共6页Journal of East China Normal University(Natural Science)

基　　金：上海市教委发展基金资助项目 (97D14)

摘　　要：从灰树花 (Grifolafrondosa)子实体和菌丝体中提取分离得到子实体多糖F -D和菌丝体多糖M -D ,得率分别是 0 .5 9%、0 .31% ;F -D的糖、蛋白质含量为 6 4 .8%、15 .6 % ,M -D糖、蛋白质含量是 6 5 .9%、7.37% ;气相色谱分析单糖组成 ,F -D由岩藻糖、阿拉伯糖、木糖、甘露糖、半乳糖、葡萄糖组成 ,各单糖的摩尔比为 0 .9:0 .5 :1.3:5 .0 :2 .9:10 0 .0 ;M -D由鼠李糖、岩藻糖、阿拉伯糖、木糖、甘露糖、半乳糖、葡萄糖组成 ,各单糖的摩尔比为 5 .9:8.5 :3.2 :2 3.2 :6 .5 :11.7:10 0 .0 ;红外光谱图显示F -D和M -D约在 890cm- 1处有吸收 ,1H -NMR谱显示两者在 5mg/L以上均无吸收 ,13C -NMR谱显示两者在 10 5× 10 - 6 、86× 10 - 6 、和 71× 10 - 6 处均有吸收 ,提示F -D和M -D多糖中均存在 β(1→ 3)和 (1→ 6 )糖苷键。Maitake polysaccharide F-D and M-D were isolated from fruit bodies and fermented mycelia of Grifola frondosa . The yield of F-D and M-D were 0.59% and 0.31% respectively. The total sugar and protein content of F-D and M-D were 64.8%?15.7% and 65.9%?7.4%. F-D was composed of Fucose, Arabinose,Xylose, Mannose, Galactose and Glucose .In the molar ratio of 0.9:0.5:1.3:5.0:2.9:100.0. M-D was composed of Rhamnose, Fucose, Arabinose, Xylose, Mannose, Galactose and Glucose. In the molar ratio of 5.9:8.5:3.2:23.2:6.5:11.7:100.0 . The infrared spectrum showed that F-D and M-D polysaccharides have absorption at 890cm -1 ; 1H-NMR spectrum showed that above 5×10 -6 there is no absorption, 13 C-NMR spectrum showed that there is obvious absorption at 105×10 -6 , 86×10 -6 and 71×10 -6 . It showed that F-D and M-D polysaccharides contain β(1→3)and (1→6) glucoside.

关 键 词：灰树花 多糖 子实体 菌丝体 真菌 D-组分 葡聚糖 

分 类 号：Q949.329.7[生物学—植物学] Q946.3