内周天线CP43、CP47中β-Car到Chla分子间的能量传递  

ENERGY TRANSFER FROM β-CAR TO CHLA IN INNER ANTENNAS,CP43 AND CP47

作　　者：贺俊芳[1] 王水才[1] 张舒[1] 贺锋涛[1] 任兆玉[1] 单际修[2] 李良壁[2] 匡廷云[2] 

机构地区：[1]中国科学院西安光学精密机械研究所瞬态室 [2]中国科学院植物所,北京100093

出　　处：《光子学报》2001年第4期385-390,共6页Acta Photonica Sinica

基　　金：国家重点基础研究专项经费!( G1 9980 1 0 1 0 0 );所长基金资助项目

摘　　要：采用超快荧光光谱动力学对从菠菜中分离纯化的内周天线 CP43、CP47进行研究 ,获取了它们的动力学三维荧光谱 ,CP43的荧光光谱范围为 640～ 780 nm,最大峰位于 680 nm处 ,在该峰值处的荧光寿命约为 3.54ns;CP47的荧光光谱范围为 630～ 775nm,最大峰位于691 nm处 ,在该峰值处的荧光寿命约为 3.2 2 ns,在 CP43和 CP47中 ,Chla分子发射荧光的效率分别约为 38.3%和 40 .6% .依据分子的退激发途径 ,我们分析认为在 CP43、CP47中 β- Car→ Chla分子的能量传递速率常量分别为 9.0 6× 1 0 11s- 1,1 .3× 1 0 12 s- 1;能量传递效率分别为47.5%、66.5% ;并估计 β- Car分子与 Chla分子外周之间的距离分别为 0 .1 1 0 nm、0 .0 85nm.CP43 and CP47,purified from spinach,were studied by using ultrafast fluorescence spectroscopy.And their dynamic fluorescence spectra were gotten.For CP47,the fluorescence spectrum range is 640～780nm and peak at～681nm with fluorescence lifetime 3.54ns.For CP47,the fluorescence spectrum range is 630～775nm and peak at～691nm with fluorescence lifetime 3.22ns.Chla emission efficiencies in CP43 and CP47 are about 38.3% and 40.6% respectively.According to molecule′s deexcitation ways,we analyzed energy transfer of β Car→Chla in CP43 and CP47 and determined that energy transfer rate constants are 9.06×10 11 s -1 and 1.3×10 12 s -1 ,energy transfer efficiencies 47.5% and 66.5% respectively.Also the distances between the surfaces of β Car and Chla in CP43 and CP47 were evaluated to be about 0.110nm and 0.085nm.

关 键 词：能量传递 超快荧光光谱 PSⅡ CP43 CP47 内周天线 β-Car Chla 菠菜 光合作用 

分 类 号：Q945.11[生物学—植物学] Q63