温度对冻存小鼠精原细胞的复苏效果  

Recovery Effect of Temperature on Cryopreserved Mouse Spermatogonia

作　　者：李莲军[1] 陆峻波[1] 施江滨[1] 李德雪[2] 张学明[2] 

机构地区：[1]云南农业大学,云南昆明650201 [2]解放军军需大学,吉林长春130062

出　　处：《云南农业大学学报》2001年第2期105-106,共2页Journal of Yunnan Agricultural University

摘　　要：采用不同温度对冻存后的小鼠精原细胞进行复苏 ,测定细胞复苏率。 4℃ ,37℃ ,67℃及 97℃时的细胞复苏率分别为 2 9 7% ,87 6 % ,88 8%及 86 4%。 37℃ ,67℃及 97℃的细胞复苏率之间在统计学上无显著性差异。结果表明 :37～ 97℃复苏小鼠精原细胞均可获得较好的复苏效果 ,而Cryopreserved mouse spermatogonia are thawed at different temperature and the recovery rates are measured. The recovery rates of mouse spermatogonia at 4?℃, 37?℃, 67?℃ and 97?℃ are 29.7%,87.6%,88.8% and 86.4% respectively. There are no significant difference between the recovery rates of 37?℃, 67?℃ and 97?℃ statistically. The results suggest that the recovery rates of mouse spermatogonia are high at 37～ 97?℃, while 4?℃ is not favorable for mouse spermatogonia recovery.

关 键 词：小鼠 精原细胞 复苏 温度 冷冻精度 

分 类 号：S814.3[农业科学—畜牧学] Q954.4[农业科学—畜牧兽医]