用RAPD技术进行鸡球虫不同种株的多态性研究  

Study of the Inter and Intraspecific Polymorphisms of Eimeria spp from the Domestic Fowl Using Random Amplified Polymorphic DNA

作　　者：陈兆国[1] 史天卫[1] 赵其平[1] 黄兵[1] 吴薛忠[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所,上海200232

出　　处：《中国预防兽医学报》2001年第5期359-363,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助项目 (30 0 0 0 12 4)

摘　　要：本文以随机引物成对组合的方式 ,对和缓艾美耳球虫 (Eimeria .mitis)、巨型艾美耳球虫 (E .maxima)和柔嫩艾美耳球虫 (E .tenella ) 3个种各 2个单卵囊感染纯株、E .tenella 6个抗药性表型不同的单卵囊感染纯株进行了RAPD分析 ,结果发现 ,RAPD技术能非常有效地用于上述球虫种、株间的鉴别。A genetic polymorphism study was performed in coccidia from the domestic fowl by random amplified polymorphic; DNA(RAPD)using pairwise combinations of primers.Two strains each of Eimeria mitis,E.maxima and six strains of E.tenella that differ markedly in drug_resistance phenotype were used.The results suggest that the RAPD assay could be an extremely useful tool for the study of differentiation of these coccidia species and strains.

关 键 词：鸡球虫 RAPD 抗药性 种株 多态性 

分 类 号：S852.723[农业科学—基础兽医学]