猪细小病毒氢氧化铝疫苗研究  

Aluminum Hydroxide Adjuvant Inactivated Vaccine to Porcine Parvovirus

作　　者：韩孝成[1] 董齐[1] 杨奇伟[1] 刘惠敏[1] 王润芝[1] 金岳[1] 李宝启[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2001年第5期381-385,共5页Chinese Journal of Preventive Veterinary Medicine

摘　　要：选用从国内浙江省某猪场流产死胎中分离的猪细小病毒强毒 (简称浙PPV_Z株 ) ,通过仔猪肾原代细胞增殖后 ,经甲醛灭活 ,再配以 2 0 %的氢氧化铝胶佐剂制成猪细小病毒氢氧化铝疫苗。该疫苗免疫源性好 ,免疫程序简单。小剂量肌注豚鼠和猪 ,均能激起明显的抗体应答反应 ,疫苗对豚鼠接种 2次HI价可达到 1∶32 0以上 (通常 1∶6 40～ 1:12 80 )。对成年猪接种疫苗 0 .2ml、0 .5ml、1.0ml免疫 2次 ,或用 2 .0ml免疫 1次 ,HI价范围在 1∶2 0～ 1∶32 0之间 ,疫苗对胎儿的保护率可达到 98.7%。实验室和田间跟踪试验显示该疫苗具有保存期长、耐热、安全、免疫保护率高等特点。Porcine Parvovirus (PPV) Z strains isolated from aborted piglets in ZheJiang province were used to develop the Aluminum Hydroxide Adjuvant inactivated Vaccine to Porcine Parvovirus, PPV were propagated in porcine primary kidney cell culture and inactivated by formaldehyde, it were mixed with Aluminum Hydroxide Adjuvant at normal rate to make the vaccine, a little dose vaccine inoculation could induce high titer antibodies in adult pig and guinea pig , HI titers were over 1:320(1∶640～1∶1280 in general) when Guinea pigs were immunized twice, HI titers could reach the rang from 1:20～1:320 when adult pigs were vaccinated twice with dose of 0.2ml?0.5ml?1.0ml respectively or once with dose of 2.0ml, immunization efficacy test of the vaccine showed that over 98.7% fetal piglets were protected. The result of lab tests and field trails indicated that the vaccine has the advantage of long period of immunity and safety.

关 键 词：猪细小病毒 疫苗 氢氧化铝胶佐剂 

分 类 号：S858.285.3[农业科学—临床兽医学] S852.659.2[农业科学—兽医学]