破伤风类毒素聚乳酸微球中蛋白质稳定性的研究  

Studies on the stability of protein in tetanus toxoid encapsulated in polylactide microspheres

作　　者：何应[1] 柳林[2] 魏树礼[1] 王东升[3] 

机构地区：[1]北京大学药学院药剂教研室,北京100083 [2]北京大学医药卫生分析中心药学与化学分析室,北京100083 [3]北京大学基础医学院免疫教研室,北京100083

出　　处：《中国药学杂志》2001年第6期391-394,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金项目 !(3 9770 878)

摘　　要：目的　研究破伤风类毒素聚乳酸微球中破伤风类毒素在制备过程和体内酸性环境中的稳定性及微球中附加剂的稳定作用。方法　采用分子排阻色谱法 ,十二烷基硫酸钠 聚丙烯酰胺凝胶电泳法 ,双向免疫扩散法。结果　在制备过程和体内酸性环境中破伤风类毒素活性有轻微下降 ;附加剂增加微球包封率 ,改善药物释放 ,稳定蛋白质活性 ,尤其是具有多羟基结构的附加剂。结论　破伤风类毒素聚乳酸微球中的蛋白质在制备过程和体内酸性环境中基本稳定 。OBJECTIVE: To investigate the stability of microencapsulated tetanus toxoid in the courses of preparation and release, and improve its stability by co-encapsulation of additives. METHODS: High-pressure size exclusion chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, double immunodiffusion were used. RESULTS: The protein denaturation processes were ascribed to the course of preparation and release. Additives, especially the additives with polyhydroxy structures, enhaned the encapsulation efficiency in the microspheres, improved the release profiles and stabilized the active proteins, respectively. CONCLUSION: The stability of tetanus toxoid loaded microspheres was satisfactory. The optimized tetanus toxoid containing microspheres may be valuable for a controlled vaccine delivery system.

关 键 词：破伤风类毒素 聚乳酸微球 蛋白质 稳定性 附加剂 

分 类 号：R944.9[医药卫生—药剂学] R927.11[医药卫生—药学]