中国对虾肝胰腺、卵巢及血淋巴中的孕酮和雌二醇含量的生殖周期变化(英文)  

Profile of progesterone and estradiol in hepatopancreas,ovary, and hemolymph of shrimp Penaeus chinensis during reproduction cycle

作　　者：蔡生力[1] 赵维信[1] 李德尚[2] 杨丛海[3] 

机构地区：[1]上海水产大学渔业学院,上海200090 [2]青岛海洋大学水产学院,山东青岛266003 [3]中国水产科学院黄海水产研究所,山东青岛266071

出　　处：《水产学报》2001年第4期304-310,共7页Journal of Fisheries of China

基　　金：国家攀登B资助计划 (PDB6 2 1)~~

摘　　要：首次证实了孕酮和雌二醇这两种类固醇激素在中国对虾体内的存在。在性腺未发育阶段 ,肝胰腺、卵巢和血液中两种激素的含量均很低 ,难以检测到。而在卵黄发生前期 (核仁周边期 ) ,三种组织中孕酮和雌二醇含量迅速上升 ,卵巢的雌二醇含量达到高峰 (45 0 .1± 86 .7)。进入初级卵黄发生阶段 ,三种组织中 ,两种激素均具较高含量 ,卵巢和肝胰腺的孕酮含量 (分别为 1975 .1± 175 .2和 90 2 .6± 130 .5pg/g)以及血淋巴中的雌二醇含量 (45 1.3± 73 .7)达到高峰。到了次级卵黄发生阶段 ,孕酮和雌二醇的含量迅速下降 ,肝胰腺等组织中几乎检测不到。对虾性腺指数 (GSI)的增长既显著且有规律性 ,每一期增长幅度都达到或超过 10 0 %。肝胰腺指数 (HSI)从性腺未发育期 (3 .4± 0 .4)到卵黄发生前期 (4.9± 0 .7)以及从卵黄发生前期到初级卵黄发生期(6 .3± 1.0 )有显著的增长 ,而从初级卵黄发生期到次级卵黄发生期HSI增长不显著 (6 .7± 1.2 )。肝胰腺指数的增长与两种类固醇激素含量的变化具相似的趋势。上述结果显示 ,孕酮和雌二醇可能具有刺激和调控中国对虾性腺发育的作用 ,肝胰腺可能是卵黄蛋白原的合成场所。The concentrations of progesterone and estradiol in hepatopancreas, ovary, hemolymph of Penaeus chinensis were investigated by means of radioimmunoassay during a reproductive cycle, and changes in GSI (gonadosomatic index) and HSI(hepatosomatic index) were monitored. Progesterone and estradiol were detected in the hepatopancreas, ovary, and hemolymph of the shrimp with maturing or matured ovary. During immature stage (length<8-10cm), the two hormones were undetectable, however, during previtellogenesis stage (perinucleolus stage, length>11-13cm), the concentrations of both hormones in three tissues increased rapidly, and estradiol in ovary reached a peak (450±86.7pg/g). During vitellogenesis stage (length>13-15cm), the two hormones maintained relatively high levels. Progesterone reached peaks in ovary (1975.1±175.2pg/g), and hepatopancreas (902.6±130.5 pg/g), and estradiol reached a peak in hemolymph (451.3±73.7 pg/ml). The hormone level decreased sharply during maturation stage, and in hepatopancreas, the hormones even became undetectable. During whole reproductive cycle, GSI increased continuously and HSI stopped increasing during maturation stage, and showed a similar change like that of hormone levels in hepatopancreas and ovary. The results suggested that hepatopancreas might be the site of vitellogenin synthesis in P. chinensis.

关 键 词：中国对虾 孕酮 雌二醇 生殖周期 性腺 血淋巴 

分 类 号：Q959.223.5[生物学—动物学] S968.22[农业科学—水产养殖]