紫细菌反应中心色素蛋白复合膜电子转移研究  

Studies on the Electron Transfer for Reaction Center Isolated from Rhodobacter Sphaeroides in Self-assembly Composite Film

作　　者：赵杰权[1] 邹永龙[2] 刘宝红[1] 彭雪松[1] 徐春和[2] 孔继烈[1] 

机构地区：[1]复旦大学化学系,上海200433 [2]中国科学院上海植物生理研究所,上海200032

出　　处：《高等学校化学学报》2001年第9期1486-1491,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :39970 195 ;2 990 5 0 0 1;39730 0 40 );国家科委重点基金 (批准号 :G19980 10 10 0 )资助

摘　　要：采用自组装单层膜方法在金电极表面形成单分子层的 2 ,3-双巯基丁二酸 ( DMSA)、聚二甲基二丙烯氯化铵 ( PDDA)及紫细菌 ( Rhodobacter sphaeroides)反应中心色素蛋白复合体的有序复合膜 .使用方波伏安法研究了该蛋白复合膜的电化学行为 ,成功地检测到该蛋白内多电对的可逆或准可逆电子转移过程 ,探讨了紫细菌反应中心蛋白内各电子受体的氧化还原电位及外加电位驱动对各受体电位的影响 .同时 ,通过对方波信号的非线形拟合 。The composite ordered protein film was assemblied on the surface of a gold electrode by self assembly mono layer approach using meso 2,3 dimercaptosuccinic acid(DMSA), poly dimethyldiallylammonium chloride(PDDA) and reaction center membrane protein purified from purple bacteria( Rhodobacter sphaeroides ). Square wave voltammetry(SWV) was employed to study the electrochemical behavior of the protein composite film. The reversible or quasi reversible electron transfer for redox couples therein was successfully detected. The reduction potential for each redox couple and the electrochemically driven effect was also discussed. The typical SWV results were non linearly fitted to get the kinetic parameters for the composite protein film.

关 键 词：电子传递 电化学 紫细菌 光合反应中心 光合作用 色素蛋白复合膜 

分 类 号：Q935[生物学—微生物学]