锇-聚乙烯吡啶复合物与辣根过氧化物酶分子多层膜的制备及其电化学性质研究  

Preparation of Ultrathin Multilayer Films Containing Poly(4-vinylpyridine) Complex of Osmium and Horseradish Peroxidase and Its Electrochemical Behavior

作　　者：李文江[1] 宋利珠[1] 孙长青[1] 王子忱[1] 郭玉鹏[1] 赵纯[1] 徐英凯[2] 

机构地区：[1]吉林大学化学系 [2]中国科学院化学研究所1北京100080

出　　处：《高等学校化学学报》2001年第9期1601-1604,共4页Chemical Journal of Chinese Universities

基　　金：吉林省自然科学基金资助

摘　　要：基于分子间静电相互作用力 ,将锇 -聚乙烯吡啶复合物 ( PVP-Os)与辣根过氧化物酶 ( HRP)交替沉积于固体基质表面 ,制得了包含生物成分的分子多层膜 .膜层间的聚合物分子起到了粘接与导电的双重作用 .用紫外 -可见光谱法跟踪了石英基片上的组装过程 ,研究了多层膜电极对过氧化氢的电催化还原性能 。We prepared a new kind of multilayer films based on ionic self assembly technique by successive alternating deposition of positively charged polycation bearing Os complex(PVP Os) and horseradish peroxidase(HRP) on the surface modified of gold electrode to study the electrochmical behavior and quartz slides to monitor the assembly process by ultraviolet visible spectroscopy. The electrochemical behavior of the enzyme electrode showed that the multilayer films were stable, electroactive, the mediator PVP Os as electron shuttle was able to transfer electrons successfully between the active center of the immobilized HRP and the electrode surface.

关 键 词：锇-聚乙烯吡啶复合物 辣根过氧化物酶 分子多层膜 电催化 还原 自组装 电化学性质 过氧化氢 玻碳电极 

分 类 号：O643.32[理学—物理化学] O646.5[理学—化学]