带萘酚基的多足胺类化合物与ctDNA的相互作用  

The Interaction between ctDNA and Multi - pedal Amino - compound with Naphthol Groups

作　　者：解宏智[1] 吴世康[1] 

机构地区：[1]中国科学院理化技术研究所，北京100101

出　　处：《化学学报》2001年第8期1222-1229,共8页Acta Chimica Sinica

基　　金：国家自然科学基金，国家重点基础研究发展计划(973计划)

摘　　要：本工作合成了一系列带萘酚基的多足胺类化合物,对它们在不同溶剂及不同pH条件下的光物理行为进行了详细研究.通过对萘酚基在不同条件下荧光光谱中存在着不同比例的萘酚基和酚负离子发光,可对化合物分子所处环境的特性作出正确的估计.工作还研究了ctDNA对该类化合物荧光的猝灭问题,指出具有三足多胺结构的该类化合物比其他两种化合物-双足及单足多胺化合物有着更强的与DNA相互作用的能力.而插入于DNA双螺旋结构的萘酚基由于能和DNA碱基对发生相互作用而引起荧光猝灭.实验结果表明:DNA的引入,对化合物的两种发光峰的猝灭有着不同的速度常数.对这一现象的机理进行了详细的讨论.A series of multi - pedal amino - compound with naphthol groups have been synthesized in this work. The photo - physical behaviors of these compounds in different solvents and different pH conditions have also been studied in details. The properties of environment in which the probe molecule located were estimated properly by the ratio of emission intensity of naphthol and phenol anion peak wave - length in the fluorescence spectra of these compounds. The problem of fluorescence quenching of the studied compounds by ctDNA was also been investigated. The results indicate that the compound with tripedal has a stronger interaction with ctDNA than that of other compounds for which one or two pedals were contained. The intercalation of naphthol group into double helix structure of ctDNA resulted in fluorescence quenching of naphthol group due to the interaction of this group and the base pair of DNA. It is due to the fact that the dual fluorescence emission of excited naphthol group had different quenching constants by ctDNA. The mechanism of this interesting phenomenon was discussed preliminarily.

关 键 词：多足荧光探针化合物 萘酚基 CTDNA 荧光猝灭 相互作用 多足胺类化合物 光物理行为 

分 类 号：Q523[生物学—生物化学] O641.3[理学—物理化学]