光谱法研究3H-吲哚探针分子与Triton X-100胶束间的相互作用  

Study on the Interactions between a 3H Indole Probe Molecule and Triton X 100 Micelles by Spectral Methods

作　　者：李健[1] 沈兴海[1] 高宏成[1] 叶建平[2] 

机构地区：[1]北京大学技术物理系,北京100871 [2]中国科学院化学研究所分子科学中心,北京100080

出　　处：《光谱学与光谱分析》2001年第4期508-511,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金 (2 990 1 0 0 1 );教育部留学回国人员科研启动基金资助项目

摘　　要：用光谱法研究了荧光分子 2 (对 己基胺基 )苯基 3,3 二甲基 5 乙酯基 3H 吲哚基 甲基二辛基碘化铵 (A)与TritonX 10 0胶束间的相互作用。测定了A在不同浓度的TritonX 10 0溶液中的吸收光谱 ,荧光光谱和荧光寿命。实验得到了A与胶束的结合常数Ks,A在胶束中所处环境的极性及其在胶束中发生的pH效应。结果表明 :A与胶束间存在较强的相互作用 ;A在胶束中所处环境的极性接近于甲醇的极性 ;并推测出了A的不同基团在胶束中所处的不同位置。同时发现 :在TritonX 10 0胶束中A可探测到两个位置 ,但当TritonX 10 0的浓度过大时 ,A却只能探测到一个位置。The interactions between a fluorescent molecule, [2-(p-hexylamino) phenyl-3, 3-dimethyl-5-ethoxycarbonyl-3H-indole] methyldiioctadecylammonium iodide (A), with Triton X-100 micelle are investigated by absorption and fluorescence spectra. The relatively large binding constant K-a shows that strong interactions between A and micelles do exist. The polarity parameter confirms that the polarity of the environment of A in the micelle is similar to that of methanol. According to the spectral characteristics and pH effects, the various sites of different groups of A in micelles are suggested. Lifetime measurements indicate that A can recognize two sites with Triton X-100 micelle at lower surfactant concentration. However, in the Triton X-100 micelle at higher surfactant concentration,A can only recognize one site.

关 键 词：3H-吲哚探针分子 TRITON X-100胶束 光谱性质 PH效应 荧光寿命 

分 类 号：O626.13[理学—有机化学] O657.3[理学—化学]