6种细胞因子对巨细胞病毒感染人胚肺成纤维细胞的影响  

EFFECTS OF SEVERAL CYTOKINES ON CYTOMEGALOVIRUS INFECTION OF HUMAN EMBRYO LUNG FIBROBLASTS

作　　者：江振友[1] 肖琼 林晨[1] 李君武[1] 曹艳英[3] 

机构地区：[1]暨南大学医学院微生物学与免疫学教研室,广州510632 [2]暨南大学医学院二附院深圳市人民医院眼科 [3]暨南大学组织移植与免疫实验中心

出　　处：《中国人兽共患病杂志》2001年第5期41-44,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助 (No 3960 0 0 0 5 );广东省医学科研基金资助 (NoA19992 80 )

摘　　要：目的　研究 6种细胞因子 (IL - 1β、IFN -α、IFN -γ、GM -CSF、TGF - β1、bFGF)对人巨细胞病毒 (HCMV)感染人胚肺成纤维细胞 (HEL)的影响 ,探讨细胞因子在HCMV感染免疫中的作用。方法　用不同浓度的 6种细胞因子分别作用于HEL ,2 4h后感染病毒 ,研究 6种细胞因子对HCMV感染HEL的影响。结果　在病毒感染前 ,IL - 1β、IFN -α、IFN -γ、bFGF作用于HEL可抑制HCMV的增殖 ;TGF - β1作用于HEL可增加HCMV的增殖 ,上述细胞因子对病毒复制的影响与细胞因子的浓度有关。GM -CSF对人巨细胞病毒感染人胚肺成纤维细胞无明显影响。结论　细胞因子在HCMV感染免疫过程中起重要作用。Aim To study the effects of interleukin-1-beta(IL-1β),interferon-alpha(IFN-α),interferon-gamma(IFN-γ),granulocyte-macrophage colony-stimulating factor(GM-CSF),transforming growth factor-beta 1(TGF-β1) and basic fibroblastic growth factor (bFGF) on human cytomegalovirus AD169 (HCMV AD169) infection in human embryo lung fibroblasts(HEL).To understand the effect of cytokines in the immune of HCMV infection.Methods IL-1β,IFN-α,IFN-γ,GM-CSF,TGF-β1,bFGF with different concentrations were incubated separately with HEL before 24 hours HCMV infection,to study the effect of six cytokines on HCMV replication in cultured HEL by cytopathic(CPE).Results IL-1β,IFN-α,IFN-γ,bFGF repress HCMV replication in cultured HEL,TGF-β1 enhances HCMV replication in HEL.The effects of Cytokines on HCMV replication were depended on their concentration,but the addition of GM-CSF showed no remarkable effect on HCMV replication.Conclusions The data suggest that Cytokines play an important role in the immune of HCMV infection.

关 键 词：人巨细胞病毒 细胞因子 人胚肺成纤维细胞 

分 类 号：R512.99[医药卫生—内科学]