基因重组融合蛋白B7-CD28共刺激阻断剂对大鼠移植肾存活的影响  

Effect of B7-CD28 costimultory blockade on the survival of rat renal allografts

作　　者：黄赤兵[1] 吴军[1] 易绍萱[1] 罗高兴[1] 张琳[1] 陈希伟[1] 周立新[1] 贺伟峰[1] 

机构地区：[1]第三军医大学西南医院烧伤研究所移植免疫室

出　　处：《中华外科杂志》2001年第7期553-555,共3页Chinese Journal of Surgery

摘　　要：目的　观察基因重组融合蛋白B7 CD2 8共刺激阻断剂CTLA 4Ig对大鼠移植肾存活的影响。　方法　肾移植术后第 2天每只用药组大鼠腹腔内注射CTLA 4Ig 0 5mg ,观察移植肾存活时间 ;术后第 2 0天 ,测定受体对供体及无关大鼠的单向混合淋巴细胞反应 (MLR) ,并观察移植肾病理改变。　结果　与对照组相比 ,用药组移植肾存活时间显著延长 [(4 2 6± 6 4)d ,(8 2± 1 2 )d ,P <0 0 0 1) ]。术后第 2 0天 ,用药组移植肾仅有散在淋巴细胞浸润 ;受体对供体的MLR明显低于正常对照[(5 832± 6 74)cpm、(134 86± 2 16 6 )cpm ,P <0 0 0 1) ],而受体对无关大鼠的MLR与正常对照相比无显著差异 (P >0 0 5 )。　结论　CTLAObjective[WT5”BZ] To observe the effect of B7-CD28 costimultory blockade CTLA-4Ig on the survival of renal allografts in rats. [WT5”HZ]Methods[WT5”BZ] CTLA-4Ig was administrated intraperitoneally at a dose of 0.5 mg to recipients of kidney transplantation in rats on the 2nd postoperative day. Renal allografts survival time and histological changes were observed, and one-way MLR of the recipients to donors and the third party rats were performed on the 20 th postoperative day. [WT5”HZ]Results[WT5”BZ] Renal allografts survival time in the CTLA-4Ig-treated group was prolonged significantly [compared with control(42 6±6 4)d vs(8 2±1 2)d, P <0 001]. On the day 20th, only sporadic lymphocyte infiltrations of the renal allografts were found in the CTLA-4Ig-treated group and CTLA-4Ig-treated recipients showed significant hyporesponsiveness to donors [compared with normal control, MLR was (5?832±674)cpm vs(13?486±2?166)cpm, P <0 001] while showed normal reaction to the third party. [WT5”HZ]Conclusion[WT5”BZ] CTLA-4Ig can prolong the survival time of rat renal allograft significantly by inducing donor-specific hyporesponsiveness. [WT5”HZ]

关 键 词：肾移植 抗体依赖细胞细胞毒性 动物实验 基因重组融合蛋白 B7-CD28共刺激阻断剂 

分 类 号：R699.2[医药卫生—泌尿科学]