粗毛栓菌产生木质纤维素酶及其降解植物生物质的研究  

Production of Lignocellulolytic Enzymes and Wheat Straw Degradation of Trametes gallic

作　　者：谢君[1] 孙迅[1] 任路[1] 张义正[1] 

机构地区：[1]四川大学生命科学学院分子生物学实验室,成都610064

出　　处：《高技术通讯》2001年第8期11-16,共6页Chinese High Technology Letters

基　　金：国家自然科学基金重点项目 (3 943 0 0 2 0 );国家教育部博士点基金资助项目

摘　　要：对在液体培养基中产生木质纤维素降解酶酶能力强且产酶速度较快的粗毛栓菌(Trametesgallic) ,进行了最佳产酶液体培养基组分的研究 ,并对其在液体培养基、固体培养基中产生木质纤维素降解酶能力和行为进行了分析。结果表明 ,该菌株在高氮低碳高无机盐培养基中的漆酶和木质素过氧化物酶的活性最高 ,在低氮高碳高无机盐培养基中的锰过氧化物酶和半纤维素酶的活性最高。该菌株培养在含有高氮无碳高无机盐和低氮无碳高无机盐液体培养基的麦草粉中 ,锰过氧化物酶在 10d、漆酶和半纤维素酶在 4 0d、木质素过氧化物酶在 5 0d时分别达到峰值 ,该菌株使麦草生物质的失重率大于 6 4 %。对半纤维素的降解达 71.96 % ,对纤维素的降解达 6 6 .2 1% ,对木质素的降解达 70 .14%。T .gallica在固体培养基中具有很强的降解生物质能力 ,且首先降解木质素。Trametes gallic was the high yield strain of lignocellulolytic enzymes and the peaks of enzyme activities were at early stage of fermentation. The optimal liquid media for the enzymes producing were defined, and the behavior of their enzymes producing was discussed. Solid state fermentation with this strain was investigated for the ability and behavior of its enzyme producing, and the results were compared with those obtained in liquid state fermentation. The work indicated that the enzyme activities of laccases, lignin peroxidases were the highest in high nitrogen, low carbon and high inorganic salt medium, and the enzyme activities of manganese dependent peroxidases, and hemicellulases were the highest in low nitrogen, high carbon and high inorganic salt medium. When T.gallic was incubated in wheat straw powder contained the liquid medium of high nitrogen, no carbon and high inorganic salt and contained the liquid medium of low nitrogen, no carbon and high inorganic salt, the activities of manganese dependent peroxidases reached the peaks on the tenth day, the activities of laccases and hemicelluloses reached the peaks on the 40th day, and the activities of lignin peroxidases reached the peaks on the50th day.,and it caused more than 64% of biomass loss,the hemicelluloses of wheat straw were degraded by 71.96%, and the cellulose was degraded by 66.21%. T. gallic was very capable of degrading lignin of wheat straw and caused 34.37% loss during 20 days, 46.71% loss during 30 days and 70.14% loss during 60 days. so it may be an excellent candidate strain in biopulping of paper industry.

关 键 词：粗毛栓菌 木质纤维素降解酶 植物生物质降解 液体培养基 酶活性 生物制浆 造纸业 

分 类 号：Q81[生物学—生物工程]