中国对虾淋巴组织培养细胞中立克次氏体形态及其感染细胞的病理观察  

Observation on Morphology of Rickettsiales and Pathology of Infected Cells in Cultured Lymphoid Tissues of the Shrimp, Penaeus chinensis

作　　者：汪岷[1] 樊廷俊[1] 郎刚华[1] 姜明[2] 童裳亮[1] 

机构地区：[1]青岛海洋大学生命学院,青岛266003 [2]青岛海洋大学测试中心,青岛266003

出　　处：《青岛海洋大学学报（自然科学版）》2001年第4期555-558,共4页Journal of Ocean University of Qingdao

基　　金：国家863课题 (819- 0 4- 0 4);高等学校骨干教师资助计划资助

摘　　要：该文报道在中国对虾淋巴组织培养细胞中发现了一种立克次氏体 ,大小 0 .2～ 0 .9μm,具有链状、双球状、亚铃状等多形性 ,存在于细胞质中。在感染细胞中有大量包涵体样的结构 ,包涵体周围包有一层膜 ,膜内存在大量不同形态、大小和电子密度的立克次氏体。在包涵体之外 ,也可观察到许多散在的立克次氏体。感染立克次氏体的细胞 ,都不同程度地出现细胞病变 ,表现为核膜溶解 ,核染色质凝聚 ,部分线粒体膜溶解 ,粗面内质网水肿。A rickettsiales organism was observed in the cytoplasm of the cultured lymphoid cells of the shrimp, Penaeus chinensis. These individual organisms were about 0.2~0.9μm and were varied in shape, such as dumbbell,diplococcus,streptococcus. Many inclusion bodies, in which the rickettsiales were varied in shape, size and electron, were observed in the infected cells. The inclusion bodies were contained by membrance . Outside the inclusion bodies, there were also many rickettsiales. Cytopathological change was observed in the infected cells, including the dissolving of nuleus membrance, chromoplasm condensing, the dissolving of the membrance of some milochondrions and edematizating of rough endoplasmis retieulum.

关 键 词：立克次氏体 中国对虾 组织培养 淋巴组织 虾病毒 病理观察 

分 类 号：S945.46[农业科学—水产养殖]