玉米矮花叶病毒HC-Pro的纯化及抗血清制备  

PURIFICATION AND ANTISERUM PREPARATION OF MAIZE DWARF MOSAIC VIRUS HC Pro

作　　者：李向东[1] 范在丰[1] 李怀方[1] 裘维蕃[1] 

机构地区：[1]中国农业大学植物保护学院植病系,北京100094

出　　处：《植物病理学报》2001年第2期135-138,共4页Acta Phytopathologica Sinica

基　　金：攀登计划! (85 - 31- 0 2 - 0 3)

摘　　要：本实验提纯了玉米矮花叶病毒 ( MDMV)的辅助成份—蛋白酶 ( HC- Pro) ,并制备了其抗血清。MDMV HC- Pro的分子量约为 57k D,提纯产量为 0 .3mg/ 10 0 g病叶。琼脂免疫双扩散测定证明MDMV HC- Pro抗血清的效价为 1∶ 16;微量免疫沉淀法测定时其效价为 1∶ 10 2 4。该抗血清只与MDMV HC- Pro有明显的反应 ,与健康玉米汁液和 MDMV无反应。MDMV HC- Pro抗血清可以专化性地抑制 HC- Pro的蚜传活性。Maize dwarf mosaic virus (MDMV) helper component proteinase (HC Pro) was purified and the specific antiserum to it was prepared in this experiment. The molecular weight of MDMV HC Pro was ca. 57 kD and its yield was 0.3 mg/100 g diseased leaves. The titer of antiserum was 1/16 in agar gel double diffusion test and 1/1024 in microprecipitation test. The antiserum reacted strongly with MDMV HC Pro, but did not with MDMV and healthy maize extracts. It could inhibit transmission efficiency of virus by aphids specifically.

关 键 词：玉米 病毒 玉米矮花叶病毒 HC-PRO 辅助成份-蛋白酶 蚜虫传毒 纯化 抗血清 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治] S432.22[农业科学—植物保护]