杂交粳稻亲本随机扩增多态性DNA(RAPD)分析(研究简报)  

RAPD ANALYSIS OF PARENTS OF HYBRID Japonica RICE

作　　者：梁婉琪[1] 黄亚红[2] 潘爱虎[1] 曾令珂[2] 张大兵[1] 袁勤[3] 全立勇[3] 曹黎明[3] 倪林娟[3] 

机构地区：[1]上海市农业科学院农业生物技术研究中心,上海市农业遗传育种重点实验室,上海201106 [2]南京大学生命科学院生物科学与技术系,南京210093 [3]上海市农业科学院作物育种栽培研究所,上海201106

出　　处：《上海农业学报》2001年第2期33-36,共4页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目!编号 :农科攻字 (97)第 7- 0 2号

摘　　要：选用 6 0个随机引物对 15份杂交粳稻亲本材料进行了RAPD分析 ,其中 10种引物的扩增结果具有明显的多态性。在这 15份杂交粳稻亲本材料中共扩增得到 153条条带 ,其中的86条为多态性条带。聚类分析结果表明 ,所有供试材料可以被明确区分。在此基础上分析了这15个水稻品种的亲缘关系 ,对田间育种有一定的参考价值。Fifteen parents of hybrid japonica rice including five male sterile lines and ten restorer lines were tested for RAPD analysis with sixty random primers. Among the rice lines tested, 153 DNA fragments were generated, of which 86 exhibited polymorphism. Cluster analysis showed that all tested lines could be clearly distinguished. Ten primers used led to the detection of obvious polymorphism among these lines. Based on these results, the evolutionary relationships of these rice lines are analyzed, and these data will provide a valuable reference for field breeding of japonica rice.

关 键 词：杂交粳稻 RAPD分析 亲本选育 

分 类 号：S511.03[农业科学—作物学]