杉木优树分子遗传变异的研究  

STUDY ON MOLECULAR GENETIC VARIATION OF SUPERIOR TREES IN CHINESE FIR(CUNNINGHAMIA LANCEOLATA(LAMB.)HOOK.

作　　者：李梅[1] 施季森[1] 何祯祥[1] 易能君[1] 

机构地区：[1]南京林业大学林木遗传和基因工程实验室,南京210037

出　　处：《林业科学》2001年第4期137-141,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金的资助 (资助号 3 9470 5 91)

摘　　要：Thirty superior trees of Chinese fir from eight provinces were analyzed on genetic variation with RAPD method.A total of 284 bands,including 190 polymorphic ones,were produced with 26 primers, and mean genetic distance between the thirty superior trees was 0.4909,indicating a high level of genetic diversity among the tress tested.Two superior trees,coded as ZJZ 01 and JXS26,showed a high value in genetic similarity,which meant that only one of them could be essentially preserved in gene bank.The clustering analysis indicated that most of the superior trees could be clustered at a relatively high genetic distance and rich genetic variation could be expected in the superior tree population.This might be valuable for marker-assisted germplasm collection,parent grouping in hybridization and genetic diversity evaluation on breeding materials in improvement of Chinese fir.Thirty superior trees of Chinese fir from eight provinces were analyzed on genetic variation with RAPD method.A total of 284 bands,including 190 polymorphic ones,were produced with 26 primers, and mean genetic distance between the thirty superior trees was 0.4909,indicating a high level of genetic diversity among the tress tested.Two superior trees,coded as ZJZ 01 and JXS26,showed a high value in genetic similarity,which meant that only one of them could be essentially preserved in gene bank.The clustering analysis indicated that most of the superior trees could be clustered at a relatively high genetic distance and rich genetic variation could be expected in the superior tree population.This might be valuable for marker-assisted germplasm collection,parent grouping in hybridization and genetic diversity evaluation on breeding materials in improvement of Chinese fir.

关 键 词：杉木 优树 遗传变异 RAPD 遗传多态性 遗传相似性 遗传距离 聚类分析 

分 类 号：S791.27[农业科学—林木遗传育种] S722.3[农业科学—林学]